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Method for detecting stroke related locus

A detection method, a stroke technology, applied in the fields of biochemical equipment and methods, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2010-06-30
SHANGHAI GENECORE BIOTECHNOLOGIES
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  • Abstract
  • Description
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Problems solved by technology

Although we can't fully say all the reasons for the decline in mortality, there is no doubt that the decline in the mortality rate of stroke patients is related to the emphasis on stroke prevention in recent years

Method used

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  • Method for detecting stroke related locus
  • Method for detecting stroke related locus
  • Method for detecting stroke related locus

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Experimental program
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Embodiment Construction

[0019] In order to better understand the content of the present invention, the following will be further described in conjunction with specific examples.

[0020] 1. Genomic DNA extraction:

[0021] 1.1 Reagents and instruments:

[0022] 1.1.1 Reagents and consumables: Genomic DNA extraction kit (TIANamp BLOOD DNA kit, DP318-03; TIANamp Micro micro sample genomic DNA extraction kit, DP316; TIANamp buccal swab genomic DNA extraction kit, DP322-03) contains : Cell Lysis Solution CL; Buffer GA; Buffer GS; Buffer GB; Buffer GD; Washing Solution PW; Elution Buffer TB; Carrier RNA; RNase-free ddH2O; Proteinase K (20mg / ml); ; Collection tube (2ML); 1.5ML sterile collection tube; Absolute ethanol.

[0023] 1.1.2 Instruments: DENVILLE260 centrifuge, XW-80A vortex mixer, H.H.S pointer electric heating constant temperature water bath.

[0024] 1.2 Extraction steps

[0025] Genomic DNA Extraction from Whole Blood

[0026] 1.2.1 Take 200 μl of anticoagulated whole blood into a 1.5ml E...

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Abstract

The invention relates to a method for detecting stroke related locus, which comprises the following steps: extracting a genome DNA of a sample from a human body; respectively designing a pair of Taqman probe pair and a pair of primer pair according to at least two stroke related genes, and respectively performing fluorescent quantitative PCR amplification, wherein the 5' end and the 3'end of the Taqman probe pair are respectively marked with a reporter and a quencher; and judging whether the stroke related genes are mutated according to a fluorescent quantitative PCR amplification result, wherein the number of the stroke related genes are preferably four, namely a MTHFR gene, an ENOS gene, a PDE4D gene and a PON1 gene. The detection method has the advantages of skillful design, simple operation and accurate and reliable detection results, and provides a reference frame for targeted health management and the subsequent detection means.

Description

technical field [0001] The present invention relates to the technical field of detection of disease-related sites, more specifically, to the technical field of detection methods for cerebral apoplexy-related sites, in particular to a detection method for cerebral apoplexy-related sites. Background technique [0002] Stroke mainly refers to the occurrence of atherosclerosis in the arteries supplying the brain, causing blockage, stenosis or rupture of blood vessels, causing damage to part of the brain tissue. It is a general term for brain diseases caused by cerebrovascular disease, so it is also called "cerebrovascular disease", "cerebrovascular accident" or "stroke". It is mainly divided into two categories: hemorrhagic stroke (cerebral hemorrhage or subarachnoid hemorrhage) and ischemic stroke (cerebral infarction, cerebral thrombosis), with cerebral infarction being the most common. Cerebral stroke has the characteristics of sudden onset and fierce onset. It has a very hi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈奕雄赵翊均
Owner SHANGHAI GENECORE BIOTECHNOLOGIES
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