Homocysteine diagnosing/measuring reagent (kit) and homocysteine concentration measuring method
A technology of homocysteine and reagents, which is applied in the field of medical inspection and measurement, and can solve the problems of large variation of test data, complicated operation, and long test time
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Embodiment 1
[0047] The homocysteine diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0048] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0049] Stabilizer 500mmol / L
[0050] Coenzyme 3mmol / L
[0051] Homocysteine desulfurase 10000U / L
[0052] Carbamyl phosphate synthase 12000U / L
[0053] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L
[0054] Sodium bicarbonate 9mmol / L
[0055] Adenosine triphosphate 5mmol / L
[0056] Glyceraldehyde-3-phosphate 5mmol / L
[0057] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0058] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of tested homocysteine sample to reagent is 1 / 25, the reaction direction is positive reac...
Embodiment 2
[0061] The homocysteine diagnosis / measurement reagent of this embodiment is a dual reagent, including:
[0062] Reagent 1
[0063] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0064] Stabilizer 50mmol / L
[0065] Coenzyme 3mmol / L
[0066] Sodium bicarbonate 9mmol / L
[0067] Adenosine triphosphate 5mmol / L
[0068] Glyceraldehyde-3-phosphate 5mmol / L
[0069] Reagent 2
[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0071] Stabilizer 50mmol / L
[0072] Homocysteine desulfurase 10000U / L
[0073] Carbamyl phosphate synthase 12000U / L
[0074] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L
[0075] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0076] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, teste...
Embodiment 3
[0079] The homocysteine diagnosis / measurement reagents of this embodiment are three reagents, including:
[0080] Reagent 1
[0081] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0082] Stabilizer 50mmol / L
[0083] Coenzyme 3mmol / L
[0084] Sodium bicarbonate 9mmol / L
[0085] Adenosine triphosphate 5mmol / L
[0086] Glyceraldehyde-3-phosphate 5mmol / L
[0087] Reagent 2
[0088] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0089] Stabilizer 500mmol / L
[0090] Carbamyl phosphate synthase 12000U / L
[0091] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L
[0092] Reagent 3
[0093] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0094] Stabilizer 500mmol / L
[0095] Homocysteine desulfurase 10000U / L
[0096] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0097] When measuring the concentration of homocy...
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