Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for inducing and acclimating epidermal stem cells into functional cells

A technology of epidermal stem cells and cells, applied in the field of induction and domestication of epidermal stem cells into functional cells, which can solve the problems of limited application range of epidermal stem cells

Inactive Publication Date: 2013-05-29
GENERAL HOSPITAL OF PLA
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The scope of application of epidermal stem cells is also limited by the influence of unipotent cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing and acclimating epidermal stem cells into functional cells
  • Method for inducing and acclimating epidermal stem cells into functional cells
  • Method for inducing and acclimating epidermal stem cells into functional cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of Epidermal Stem Cells Before Induction and Domestication

[0027] 1. Preparation of epidermal stem cells:

[0028] Direct source and original source: With the consent of the patient, it was collected from healthy circumcision patients in the Surgical Outpatient Department of 301 Hospital.

[0029] (1) Take fresh adult foreskin (in vitro time is about 1-4 hours, stored at 4°C);

[0030] (2) soak the foreskin in dilute iodophor solution (containing iodophor 20%) for 1 min;

[0031] (3) Rinse with PBS / normal saline for 3 times to remove blood clots (to prevent affecting the digestion of enzymes);

[0032] (4) Trim the foreskin, remove the fat tissue, rinse with PBS / saline several times, and finally cut into 1cm 2 left and right organizational blocks;

[0033] (5) Place the tissue block in a 6cm dish containing neutral protease (3mg / ml; D-hanks as solvent) solution and treat it at 4°C for 14-16h. If the treatment is not enough, it can also be pla...

Embodiment 2

[0108] Example 2 Induction and domestication of epidermal stem cells into functional cells (nerve cells, fat cells and muscle cells)

[0109] 1. Culture medium composition

[0110] (1) The composition and dosage of non-directional induction medium are: 89% DMEM / F12 (Gibico), 10% FBS and 1% PS (double antibody Invitrogen, cat. no. 15070).

[0111] (2) The composition and dosage of the dry maintenance medium are: 98% Epilife (Cascade Biologics, cat.no.M-EPI-500-CA), 1% PS (double antibody, Invitrogen, cat.no.15070) and 1% HKGS Supplement (HKGS, Cascade Biologics, cat. no. S-001-5).

[0112] 2. Induced domestication method:

[0113] (1) Pre-treatment for domestication induction:

[0114] The cells need to be washed with PBS 1-2 days before the start of cell induction, in order to determine the number of small round cells and replace with fresh Epilife medium.

[0115] (2) After the epidermal stem cells are cultured, take epidermal stem cells with a density greater than 60% th...

Embodiment 3

[0118] Identification and result analysis of the target cell of embodiment 3

[0119] (1) Identification method:

[0120] In the experimental verification of induction into muscle cells, nerve cells and adipocytes, muscle cell markers (a-SMA) and nerve cell markers (Nestin) were detected by immunofluorescence, and oil red O staining of adipocytes was proved positive by oil red staining experiments .

[0121] Muscle cells: detect a-SMA by immunofluorescence within 1-3 weeks after the end of acclimatization induction;

[0122] For nerve cells, Nestin was detected by immunofluorescence within 1-3 weeks after the end of domestication induction;

[0123] Adipocytes: Stain with Oil Red O within 1-3 weeks after the end of acclimation induction: Take the cells after induction (observe the morphology of adipocytes under a microscope, and there are many lipid droplet vacuoles in the cells), wash the cells 1-3 times with PBS , add the prepared oil red O dye solution (oil red: distille...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for inducing and acclimating epidermal stem cells into functional cells, comprising the following steps of: mixing an eplife culture medium and an induction culture medium according to different proportions, and ensuring that the epidermal stem cells have enough time fit for the continuous reduction of the eplife culture medium and the gradual increase of the induction culture medium, which is beneficial to reducing the differentiation to the epidermis and increasing the transformational probability to targeted cells. The method is simple and very effective tosimultaneously induce and acclimate the epidermal stem cells into nerve cells, fat cells and muscle cells in a nondirective way, overcomes the prejudice from technical personnel in the filed, subverts a judgment that the epidermal stem cells are unipotent stem cells for the first time, and proves that the epidermal stem cells have the multi-directional differentiation potential.

Description

technical field [0001] The invention relates to a method for inducing cells, in particular to a method for inducing and acclimating epidermal stem cells into functional cells, and the functional cells are mainly nerve cells, fat cells and muscle cells. Background technique [0002] Humans have been studying epidermal stem cells for decades, but due to their difficulty in inducing differentiation, they have always been considered as unipotent stem cells, that is, they can only differentiate into related components of the epidermis. Regenerative medicine is hot in the field of medicine, and the research on stem cells has occupied an important position in the field of regenerative medicine. Stem cells, due to their multi-differentiation potential, can differentiate into various tissues and organs under specific conditions have attracted the attention of scientists. . Embryonic stem cells are totipotent stem cells that can differentiate into all tissues and organs except the pl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08
Inventor 付小兵陈美霞韩为东
Owner GENERAL HOSPITAL OF PLA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com