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A kind of laurel wood extract and its preparation method and application

A technology of extract and white osmanthus, which is applied in the field of medicine and food, can solve the problems of induced skin diseases, cytotoxicity, weak whitening effect, etc., and achieve the effect of preventing skin aging and protecting skin

Inactive Publication Date: 2011-12-14
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a variety of tyrosinase inhibitors have been used for whitening, there are still problems and cannot meet the needs of the consumer market
Kojic acid has weak whitening effect due to its instability; hydroquinone compound has a strong tyrosinase inhibitory effect, but because it can induce skin diseases and cytotoxicity, many countries have banned its use; arbutin is relatively safe, but its effect is weak

Method used

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  • A kind of laurel wood extract and its preparation method and application
  • A kind of laurel wood extract and its preparation method and application
  • A kind of laurel wood extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] After 20 kg of dry stems and branches of Osmanthus osmanthus were crushed, soaked in 100 liters of 95% (v / v) ethanol for 12 hours, then percolated, collected 400 liters of percolation liquid, concentrated and dried to obtain 1.8 kg of extract. Dissolve the extract in distilled water and then extract with chloroform and ethyl acetate respectively, take the ethyl acetate solvent extraction phase, recover the ethyl acetate solvent and concentrate to dryness to obtain 500 grams of Osmanthus osmanthus extract of the present invention. Measured by high-performance liquid chromatography, the chromatographic conditions are: the chromatographic column is a HyperClone BDS C18 (4.6mm × 250mm, 5 μm) column, the mobile phase is 70% (v / v) methanol-0.2% phosphoric acid aqueous solution, and the detection wavelength is 300nm. The sum of the contents of flavonoids artopetelin B, artonin A, artocarpin and brosimone I substituted by isopentenyl in the extract is 40% by weight.

Embodiment 2

[0039] After 20 kg of dried stems and branches of Osmanthus osmanthus were crushed, soaked in 100 liters of 70% (v / v) ethanol for 12 hours, then percolated, collected 400 liters of percolation liquid, concentrated and dried to obtain 1.5 kg of extract. Dissolve the extract in distilled water and then extract with chloroform and ethyl acetate respectively, take the ethyl acetate solvent extraction phase, recover the ethyl acetate solvent and concentrate to dryness to obtain 450 grams of Osmanthus osmanthus extract of the present invention. Determined by high performance liquid chromatography, the chromatographic conditions are: the chromatographic column is HyperClone BDS C 18 (4.6mm × 250mm, 5 μm) column, mobile phase is 70% (v / v) methanol-0.2% phosphoric acid aqueous solution, detection wavelength is 300nm, the flavonoids artopetelin B, artonin A that isopentenyl replaces in this extract The sum of the contents of , artocarpin and brosimone I is 34% by weight.

Embodiment 3

[0041] After 20 kg of dry stems and branches of Osmanthus osmanthus were crushed, soak them in 100 liters, 80 liters, and 60 liters of 80% (v / v) aqueous methanol at room temperature for three times, for 24 hours for the first time, and 12 hours for the second and third times respectively. hours, the combined extracts were concentrated and dried to obtain 1.2 kg of extract. Dissolve the extract in distilled water and then extract it with chloroform and ethyl acetate respectively, take the ethyl acetate solvent extraction phase, recover the ethyl acetate solvent and concentrate to dryness to obtain 420 grams of Osmanthus osmanthus extract of the present invention. Determined by high performance liquid chromatography, the chromatographic conditions are: the chromatographic column is HyperClone BDS C 18(4.6mm * 250mm, 5 μ m) column, mobile phase is 65% (v / v) acetonitrile-0.2% phosphoric acid aqueous solution, detection wavelength is 300nm, the flavonoid compound artopetelin B, art...

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Abstract

The invention belongs to the technical fields of medicaments and foods and relates to an artocarpus hypargyreus hance extract and a preparation method and application thereof. In the artocarpus hypargyreus hance extract, the content of an isopentenyl-substituted flavonoid compound is 20 to 40 weight percent. The preparation method comprises the following steps; extracting stems and branches of artocarpus hypargyreus hance serving as a raw material by a solvent to prepare a total extract; dissolving the total extract in water, extracting with a halogenated solvent and an ester solvent in sequence; and taking an extraction phase of the ester solvent, and drying after reclaiming the solvent to obtain the artocarpus hypargyreus hance extract. Through activity tests such as tyrosinase in vitro, B16 melanoma cells, DPPH clearing and the like, results show that the artocarpus hypargyreus hance extract has strong effects of inhibiting the generation of the tyrosinase and melanin and clearing free radicals, can be used as a melanin inhibitor, a whitening agent or a natural antioxidant, and is applied to the fields of medicament, daily chemical engineering and food.

Description

technical field [0001] The invention belongs to the technical field of medicine and food, and relates to a laurel wood extract and its preparation method and application. Specifically, it relates to an ethyl acetate extract of laurel wood, a preparation method thereof and its use as a melanin production inhibitor or a natural antioxidant. Background technique [0002] With the improvement of aesthetic standards, people's requirements for whitening and delicate skin are increasing day by day. In recent years, the development of skin whitening and anti-aging cosmetics market has been paid more and more attention. Whitening and anti-aging cosmetics have become one of the mainstream varieties of skin care cosmetics, and their consumer market is constantly expanding. [0003] The color of human skin depends on the content and distribution of melanin. Skin hyperpigmentation is caused by excessive production of melanin in melanocytes, and tyrosinase is an essential key enzyme in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K31/343A61K36/60A61K31/35A61P17/00A61P39/06A61K8/97A61Q19/02A61Q19/08A23L1/28A23L1/30A61K135/00A23L33/105
Inventor 王贺瑶侯爱君闫桂蕊赵婷乔欣
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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