Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Magnetic relaxation switch based on Fe304@Au and detection method thereof

A switching and magnetic technology, applied in the field of magnetic relaxation switches and their preparation, can solve problems such as insufficient concentration correlation, and achieve the effect of low detection limit and uniform particle size

Inactive Publication Date: 2010-07-14
FUDAN UNIV
View PDF8 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] MRSw technology is currently in its infancy, and despite the obvious practical prospects, the T of MRSw 2 The correlation between the signal change and the concentration of the analyte is not stable enough, and the T 2 The signal has completely different changing trends before and after the concentration of the analyte reaches the highest value of the detection range. It is necessary to use other detection methods to supplement and demonstrate the relaxation switch technology, and to improve the practical application value of MRSw in the way of multiple detection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Magnetic relaxation switch based on Fe304@Au and detection method thereof
  • Magnetic relaxation switch based on Fe304@Au and detection method thereof
  • Magnetic relaxation switch based on Fe304@Au and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the nanometer Fe of synthetic dextran wrapping 3 o 4

[0025] Add 4 mL of concentrated NH to 15 mL of dextran aqueous solution (15% w / w, Mw=20000) at room temperature 4 OH (>25% w / w) to make the pH of the solution about 11.7, and then heated to 25° C. in a water bath under magnetic stirring. Put 0.75gFeCl 3 ·6H 2 O and 0.28 g FeSO 4 4H 2 O was dissolved in 5 mL of water, filtered through a 0.22 μm filter membrane and added dropwise to the dextran solution, the solution turned into a black turbid liquid, and stirred for 0.5 h. Afterwards, the turbid solution was centrifuged at 10,000 rpm for 20 minutes to remove larger particles, and the upper orange-red clear solution was dialyzed with deionized water for 24 hours (using a dialysis bag of 25 kDa) to remove excess ammonium ions and free dextran in the solution. Afterwards, the above solution was concentrated by centrifugal ultrafiltration. TEM images showing the resulting Fe 3 o 4The nanoparticle ...

Embodiment 2

[0026] Embodiment 2: synthetic flower shape Fe 3 o 4 @Au magnetic nanoparticles

[0027] Take 5mL of the above concentrated Fe 3 o 4 Nanoparticle solution, add 4 mL of deionized water, add 200 μL of NH under magnetic stirring 2 OH (w / w=1%) aqueous solution, stirred for 10min, added 0.5g glucose as reducing agent, then added 50μL of NH 4 OH (7%), make the pH of the solution about 9.0, then add 100 μL of 0.25% HAuCl in 4 times 4 Aqueous solution, a total of 400μL was added, with an interval of 10min between each two, with HAuCl 4 The solution gradually changed from reddish brown to dark red. Afterwards, centrifuge at 7000rpm for 6min, remove the supernatant, and redissolve the lower sediment in water, and then undergo repeated ultrasonic dispersion (80W) and centrifugal separation and washing for 5 times to remove Fe that is not wrapped with Au. 3 o 4 , and finally dissolved in 1% dextran solution, the solution was dark blue and could remain stable within a week. The TE...

Embodiment 3

[0028] Example 3: In Fe 3 o 4 Surface modification of @Au magnetic nanoparticles with biotin

[0029] First self-assemble cystamine on the particle surface: take 2mL of the above Fe 3 o 4 @Au solution was centrifuged at 7000rpm for 6mins to remove the supernatant, and 2mL of 1mM PBS (pH=8.0) was added, and cystamine solution was added while sonicating to make the final concentration 4mM. Continue sonicating for 10min, then magnetically stirred for 8h, centrifuged again and used Washed three times with deionized water, and finally ultrasonically dispersed in 5 mL of 1 mM PBS (pH=8.0) solution, and 200 μL of 5% dextran solution was added to improve the stability of the solution.

[0030] Then connect Biotin through cystamine: dissolve 2mg Biotin, 10mg EDC, 1mg NHS in 2mL of 2mM PBS (pH=7.4) buffer, shake gently for 10mins, and then add to the above Fe 3 o 4 @Au solution, magnetically stirred for 12 hours, centrifuged at 7000rpm for 6 minutes, removed the supernatant, washed...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of biological detection, and particularly discloses a magnetic relaxation switch based on Fe304@Au and a detection method and detection application thereof. In order to solve the problems of inconvenience and unstable detection results of the technical embodiment of a traditional magnetic relaxation switch, the invention develops a magnetic relaxation switch detection technology with a flower-like Fe304@Au core-shell structure magnetic nanoparticle as a probe. By modifying and identifying biotin molecules on a gold layer on the surface of the nanoparticle, the invention realizes the quick and convenient detection of model protein Avidin, and the detection limit is low to the level of 10nM.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and specifically relates to providing a method based on Fe 3 o 4 Magnetic relaxation switch of @Au magnetic nanoparticles and its preparation method and detection application. Background technique [0002] The biosensor based on superparamagnetic nanoparticles is a high-efficiency detection technology developed in recent years. It breaks through the limitation of magnetic resonance in vitro application. This technology is called magnetic relaxation switch technology (Magnetic RelaxationSwitch). In an external magnetic field, the magnetic dipole of the paramagnetic particle itself will generate a small magnetic field disturbance around it, causing 1 H nuclear spin degenerates, such that detected by radio wave pulse train 1 The H signal will weaken, and the time constant determined by the obtained relaxation exponential degradation curve is T 2 , when the target molecule appears in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01R33/465G01N33/50G01N33/68B22F9/24
Inventor 孔继烈梁国海陈惠
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com