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Multi-functional biological agent containing bacillus subtilis

A technology of Bacillus subtilis and biological agents, applied in the field of multifunctional biological agents, to achieve the effects of simple manufacturing process, solving soil compaction and heavy cropping diseases, and good adsorption

Inactive Publication Date: 2010-08-04
山东多利德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Soil compaction and cropping diseases often go hand in hand, but so far there is no effective method that has the effect of soil improvement and cropping disease control at the same time

Method used

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  • Multi-functional biological agent containing bacillus subtilis
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  • Multi-functional biological agent containing bacillus subtilis

Examples

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example 1

[0042] Example 1, soil improvement of the present invention, anti-heavy cropping multifunctional biological preparation is formulated from the following raw materials by weight (parts):

[0043] Bacillus subtilis fermentation broth 42, fulvic acid 15, zinc sulfate 4, vitamin C 7, α-naphthyl acetate 0.8, chlorogenic acid 10, aminosalicylic acid 7, dextrin 8, add water to 100 parts.

example 2

[0044] Example 2, soil improvement of the present invention, anti-heavy cropping multifunctional biological preparation is formulated from the following raw materials by weight (parts):

[0045] Bacillus subtilis fermentation broth 40, fulvic acid 10, zinc sulfate 3, vitamin C 6, α-naphthyl acetate 0.7, chlorogenic acid 9, aminosalicylic acid 6, dextrin 7, add water to 100 parts.

example 3

[0046] Example 3, soil improvement of the present invention, anti-heavy cropping multifunctional biological preparation is formulated from the following raw materials by weight (parts):

[0047] Bacillus subtilis fermentation broth 45, fulvic acid 20, zinc sulfate 5, vitamin C 7, α-naphthyl acetate 0.8, chlorogenic acid 9, aminosalicylic acid 6, dextrin 8, add water to 100 parts.

[0048] The preparation method of soil improvement and anti-heavy cropping multifunctional biological preparation of the present invention:

[0049] ①Preparation method of Bacillus subtilis fermentation broth: (Sterile operation should be performed during processing)

[0050] The Bacillus subtilis strains were activated on LB plates, placed in liquid LB medium (tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L), and cultured on a shaking table at 28°C and 120rpm for 24h According to 3% inoculation amount, carry out agitation and aeration culture in the seed tank for 24 hours, then enter the secondary s...

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Abstract

The invention provides bacillus subtilis DLD2 bacterial strain which is preserved in a China general microbiological culture collection center (CGMCC), the serial number is CGMCC NO.3222. The invention further provides a multi-functional biological agent formula and a preparation method thereof; fermentation broth, fulvic acid, zinc sulfate and other components of the bacillus subtilis DLD2 are formulated for preparing the multi-functional biological agent formula which is used for improving soil and continuous cropping resistance; the biological agent has the following functional effect: (1) bio-control microorganisms are utilized to prevent harmful flora from infecting plant and killing pathogenic bacteria, induce the plant to generate disease resistance and prevent seedling withering and rotting; (2) by utilizing synergia effect of formulation of the bacillus subtilis, fulvic acid and zinc sulfate, the root system growth can be induced, plant regeneration and root regeneration are realized, soil hardening is broken, granular structure is formed, no deep tillage is realized, moisture holding and soil moisture conservation is realized, fertilizer damage is relieved and soil fertility is improved.

Description

technical field [0001] The invention is a multifunctional biological agent containing bacillus subtilis, and relates to the formula and preparation method of soil improvement and anti-stubble agent. Background technique [0002] Good soil conditions are a necessary condition for high crop yields, but due to drought, excessive use of chemical fertilizers, unreasonable cultivation and management, continuous cropping and other reasons, soil ecology deteriorates, crop production decreases, and huge economic losses are caused. The main impacts of soil ecological deterioration on crops are root water, vapor, and fertilizer imbalances caused by soil compaction and heavy cropping diseases caused by heavy cropping pathogens. Exploring effective methods to break soil compaction and control cropping diseases has huge economic benefits and social significance. [0003] At present, the methods for breaking soil compaction are mainly mechanical deep plowing and application of soil amendm...

Claims

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Application Information

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IPC IPC(8): C09K17/14A01N63/02A01N61/00A01P1/00A01P21/00C05G3/00C12N1/20C12R1/125C09K101/00
Inventor 宋保平陈靠山尹倩倩孙斐李淑梅
Owner 山东多利德生物科技有限公司
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