Method for detecting induced pluripotent stem cell
A technology of pluripotent stem cells and silicon spheres, which is applied in the field of bioengineering, can solve the problems of low fluorescence intensity of organic fluorescent dyes, high toxicity of organic fluorescent dyes, difficult determination results, etc., achieves good biological safety, and is less likely to be interfered. , The effect of low detection cost
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Embodiment 1
[0025] Detection of specific oct4, sox2, nanog, and AP antigens expressed on the surface of iPS cells
[0026] The detection method is to analyze the expression of oct4, sox2, nanog, and AP antigens in ips cells. Human oct4, sox2, nanog, and AP monoclonal antibodies are used as detection methods, anti-BSA monoclonal antibody is used as a negative control, and anti-beta-tubulin monoclonal antibody is used as a negative control. positive control.
[0027] Step 1, weigh 1.35g (0.005mol) FeCl 3 ·6H 2 O and 0.69 (0.0025mol) FeSO 4 ·7H 2 O was dissolved in 50ml of deionized water, and 20ml of 1.5M NaOH was added under nitrogen protection at 80°C. After vigorously stirring for 1 hour, the black precipitate was separated by magnetic separation, and washed three times with deionized water. Obtain magnetic nanoparticles, then disperse the magnetic nanoparticles in 20ml deionized water to form a solution with a concentration of 8mg / ml;
[0028] Step 2, take 1.1417g (5mmol) CdCl 2 2.5...
Embodiment 2
[0034] Detection of specific SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 antigens expressed on the surface of ips cells
[0035] The detection method is to analyze the expression of SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 antigen in ips cells, select anti-SSEA-3, SSEA-4, TRA-1-60, TRA-1- 81 monoclonal antibody was used as a detection method, anti-BSA monoclonal antibody was used as a negative control, and anti-GAPDH monoclonal antibody was used as a positive control;
[0036] Step 1, weigh 1.35g (0.005mol) FeCl 3 ·6H 2 O and 0.69 (0.0025mol) FeSO 4 ·7H 2 O was dissolved in 50ml of deionized water, and 20ml of 1.5M NaOH was added under nitrogen protection at 80°C. After vigorously stirring for 1 hour, the black precipitate was separated by magnetic separation, and washed three times with deionized water. Obtain magnetic nanoparticles, and finally disperse the magnetic nanoparticles in 20ml deionized water to form a solution with a concentration of 8mg / ml;
[0037] Step 2, 1.1417g (5mmol...
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