Use of cordyceps species or extract thereof in the egg production
A kind of Cordyceps fungus, Cordyceps fungus technology, applied in the direction of application, medical preparations containing active ingredients, animal feed, etc.
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Embodiment 1
[0053] The extract of at least one species of Cordyceps is preferably cultured according to the method described in published Singapore Patent Application No. 200400686-2 (Singapore Patent 129276). The FIRDI-numbered Cordyceps strain was obtained from the Taiwan Food Industry Development Institute.
[0054] Described below are the production methods of different Cordyceps strains. It should be noted that growth parameters, such as fermentation time, temperature, agitation speed, aeration, pH range, etc., are not necessarily limited to the values shown, but may vary by a reasonable amount, as generally understood by those skilled in the art.
[0055] Cultivation of Cordyceps sinensis in a fermenter:
[0056] Glucose, maltose or powder, yeast extract raw materials and food-grade chemicals listed in Table 1 are used to prepare a liquid medium containing a carbon source, a nitrogen source, vitamins, minerals and other growth factors. To produce a Cordyceps fungus, such as Cord...
Embodiment 2
[0094] The extraction process of embodiment 2 Cordyceps bioactive components
[0095] According to traditional methods of known technology, the bioactive components of Cordyceps can be extracted into water-soluble and ethanol-soluble compounds. However, these traditional methods of extraction do not include hot water extraction, which is described in more detail below. A traditional method without hot water extraction is ineffective in extracting water-soluble polysaccharides. Hot water can also be adjusted to pH 10 with buffers to further enhance efficacy.
[0096] Soak 1,700 grams of wet Cordyceps mycelium in 6 liters of hot water (about 90°C) overnight with gentle stirring for about 24 hours or more. After the Cordyceps fungus is filtered, the filtrate is mixed with 2 volumes of 99% ethanol (1 volume is 6 liters). The filtrate was removed by filtration and the precipitate caused by ethanol was collected. The precipitate was washed with 50% ethanol and then freeze-dried t...
Embodiment 3
[0102] The preparation of real test example 3 cordyceps slurries
[0103] After completing the large-scale cultivation method described in Example 1, the mycelium is not collected and dried, but collected through a needle-punched polyester filter medium, which is filtered by gravity or by a controlled vacuum force, so that the wet fungi The filaments contained 10-20% total solids. The wet mycelium was rinsed twice with water to remove residues.
[0104] The range of extraction rate of Cordyceps slurry is 300-500 kg per cubic meter of fermented liquid.
[0105] The wet mycelia at 10-20% total solids is placed in a colloid mill or homogenizer or any suitable type of mill for homogenization. After homogenization, a wet mycelium with a particle size of 0.5-3.0 microns can be obtained, which allows the mycelium to be well dispersed in the liquid. The obtained Cordyceps fungus preparation is called "Cordyceps fungus slurry".
[0106] The specific characteristics of the obtained ...
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