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Cultivate method for cordyceps militaris link

A cultivation method, the technology of Cordyceps militaris, which is applied in the field of cultivation of Cordyceps militaris, can solve the problems of affecting the output, reducing the quality, improper deployment, etc., and achieve the effects of shortening the maturity cycle, improving labor efficiency and ensuring full absorption

Inactive Publication Date: 2008-05-07
袁有宝
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a uniform temperature is given to the above-mentioned period, or if the temperature is different, but it does not fully meet its growth needs, it will result in a decrease in the yield and quality of the fruiting bodies.
[0006] 2. The proportion of raw materials such as grains and water in the grain medium is improperly prepared
There are generally too many liquid strains in the existing medium, and too many liquid strains in the medium will cause excess nutrition on the surface of the medium, dense strains, and excessive growth, resulting in mycelium on the surface of the medium in a short period of time. Thickened mycelium, the thickened mycelium is pressed on the surface of the culture medium like a layer of quilt
Since the fruiting body is formed in the thinner mycelium on the surface of the medium, it is difficult for the thicker mycelium to produce sporocarps to form the fruiting body, so that the fruiting body is produced less and cannot reach the fruiting body on the surface of the medium full germination, and affect the subsequent yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, this embodiment includes the following steps:

[0038] a. Cultivate liquid strains;

[0039]According to the ratio of water and raw materials, the medium is prepared according to the following formula: glucose 5-6%, yeast extract 1-1.5%, peptone 1-1.5%, potassium dihydrogen phosphate 0.1-0.15%, vitamin B10.005-0.010%, Pour the prepared liquid culture medium into a culture bottle and carry out high-pressure steam sterilization at 121°C for 30 minutes. After cooling, insert the prepared pure strains with good stability and cultivate them for 5 days to obtain liquid strains.

[0040] B, in the allotment rice solid culture medium in culture bottle;

[0041] The present invention selects 500ml culture bottle, packs rice 33-38g, silkworm chrysalis powder 1-3g, prepares the following nutrient solution formula: glucose 2.5-3%, peptone 0.05%-1.5%, dipotassium hydrogen phosphate 0.2%-0.5%, diphosphate Potassium Hydrogen 1%-1.5%, Triamine Citrate 0.5%-1%, Vitamin B...

Embodiment 2

[0056] Embodiment 2, this embodiment includes the following steps:

[0057] a. Cultivate liquid strains;

[0058] According to the ratio of water and raw materials, prepare the medium according to the following formula: glucose 5-6%, yeast extract 1-1.5%, peptone 1-1.5%, potassium dihydrogen phosphate 0.1-0.15%, vitamin B 1 0.005-0.010%, pour the prepared liquid culture medium into the culture bottle and carry out high-pressure steam sterilization at 120°C for 29 minutes. After cooling, insert the prepared pure strains with good stability and cultivate them for 4 days to obtain liquid strains.

[0059] B, in the allotment millet solid culture medium in culture bottle;

[0060] The present invention selects 500ml culture bottle, packs millet 33-38g, silkworm chrysalis powder 1-3g, prepares following nutrient solution formula: glucose 2.5-3%, peptone 0.05%-1.5%, dipotassium hydrogen phosphate 0.2%-0.5%, diphosphate Potassium Hydrogen 1%-1.5%, Triamine Citrate 0.5%-1%, Vitamin ...

Embodiment 3

[0074] Embodiment three, the present embodiment is cultivated according to the following steps:

[0075] a. Cultivate liquid strains;

[0076] According to the ratio of water and raw materials, prepare the medium according to the following formula: glucose 5-6%, yeast extract 1-1.5%, peptone 1-1.5%, potassium dihydrogen phosphate 0.1-0.15%, vitamin B 1 0.005-0.010%, pour the prepared liquid culture medium into a culture bottle and carry out high-pressure steam sterilization at 123°C for 31 minutes. After cooling, insert the prepared pure strains with good stability and cultivate them for 6 days to obtain liquid strains.

[0077] b. Prepare corn solid culture medium in a culture bottle;

[0078] The present invention selects 500ml culture bottle, packs corn 33-38g, silkworm chrysalis powder 1-3g, prepares the following nutrient solution formula: glucose 2.5-3%, peptone 0.05%-1.5%, dipotassium hydrogen phosphate 0.2%-0.5%, diphosphate Potassium Hydrogen 1%-1.5%, Triamine Citra...

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Abstract

The invention relates to a cultivation method of Cordyceps militaris. In the method, the inoculated grain culture medium is placed in a cultivation room for cultivation, and the cultivation process is divided into two periods: a mycelium growth period and a sporocarp growth period. The growth characteristics give it the temperature environment that it needs to grow and increase step by step, so as to shorten the cultivation period and obtain high-quality and high-yield Cordyceps militaris.

Description

technical field [0001] The invention relates to a cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris is a precious Chinese medicinal material with high medicinal and nourishing effects. The distribution of wild Cordyceps is scarce and resources are extremely scarce. In order to meet the market demand, artificially cultivated Cordyceps militaris is currently used. The artificially cultivated Cordyceps militaris is also called Cordyceps militaris. Its active ingredients are close to natural ones, and the content of some ingredients is even several times or dozens of times higher than natural Cordyceps sinensis. [0003] At present, many domestic and foreign companies are engaged in the research work of artificial cultivation of Cordyceps militaris, in order to obtain the best natural law of growth of artificial cultivation of Cordyceps, and strive to cultivate fruiting bodies of Cordyceps with higher quality and higher yield, and a num...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 孙婵娟袁博阳袁有宝
Owner 袁有宝
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