Photocrosslinking nano hydrogel and preparation method thereof

Abstract

The invention relates to a photocrosslinking nano hydrogel which comprises the effective component of an ultraviolet photo-initiated azido crosslinking carboxymethyl chitosan derivative, and is prepared by the method comprising the following steps: taking the water solution of carboxymethyl chitosan; adding the organic solution of 4-azidobenzaldehyde under the condition that the mol ratio of the sugar moiety of the carboxymethyl chitosan to the 4-azidobenzaldehyde is 1:(0.01-10); stirring and mixing evenly at 0-30 DEG C under the dark condition, wherein when a photocrosslinking carboxymethyl chitosan amphiphilic polymer is generated by the reaction, the amphiphilic polymer can be self-assembled into a photocrosslinking nano-particle mixed solution; and then radiating the nano-particle mixed solution by ultraviolet light to initiate azido crosslinking. The invention has simple process and easy operation, can obtain photocrosslinking targeted nano gel by coupling carboxyl with a target molecule or a target molecule derivative, and has potential application value in the fields of tissue engineering, biomedical implants, biological nano technology, drug delivery systems, and the like.

Description

technical field [0001] The invention relates to a gel material, in particular to a method for preparing a photocrosslinked nano hydrogel. Background technique [0002] Nanogel is a microgel with a size ranging from 10 to 1000 nanometers. It not only has high water content, good biocompatibility and adjustable chemical and mechanical properties, but also high specific surface area can couple biomacromolecules, cross-linked network can coat therapeutic molecules, and adjustable particle size facilitates regulation its distribution in the body. These properties lead to the wide application potential of nanohydrogels in tissue engineering, biomedical implants, bionanotechnology and drug delivery systems. Although there are many methods for preparing nanogels, and different methods produce gels with different particle sizes and surface structures, resulting in different uses, they can still be divided into two methods: physical and chemical. The physical method is to use hydro...

AI Technical Summary

Problems solved by technology

[0006] (1) In-situ drug loading cannot be achieved for drugs or active molecules with amine groups, because the cross-linking agent is easy to chemically react with the amine groups on the drug or active molecules

[0007] (2) The preparation process is more complicated, and toxic cross-linking agents such as glutaraldehyde must be removed by dialysis and other methods

[0009] Although the physical cross-linking method can avoid some defects of the chemical cross-linking method, its stability, especially in the internal environment of the human body, is not as good as that of the chemical cross-linking method, which will lead to premature rupture of the nanogel, and premature release of the drug or active agent. freed

In addition, the nanogel obtained by physical cross-linking has a wide particle size distribution and is easy to aggregate.

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