Rhizobiumrhizogenes strain, bactericide and application thereof serving as seedling raising matrix in preventing and controlling tomato bacterial wilt

A technology of Rhizobium rhizobia and tomato bacterial wilt, applied in the biological control of tomato bacterial wilt, in the field of biology, can solve the problems of long crop rotation, difficult implementation, infeasibility, etc., achieve low cost, avoid secondary pollution, Vibrant effect

Inactive Publication Date: 2010-09-01
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The paddy and dry rotation requires a long rotation period and no other hosts nearby to achieve good results, so it is difficult to implement
After the soil is sterilized, a soil biological vacuum is formed. Once the soil is infected by pathogenic bacteria, it will lead to a large outbreak of pathogenic bacteria and cause more seri

Method used

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  • Rhizobiumrhizogenes strain, bactericide and application thereof serving as seedling raising matrix in preventing and controlling tomato bacterial wilt
  • Rhizobiumrhizogenes strain, bactericide and application thereof serving as seedling raising matrix in preventing and controlling tomato bacterial wilt
  • Rhizobiumrhizogenes strain, bactericide and application thereof serving as seedling raising matrix in preventing and controlling tomato bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1: Obtaining of Rhizobium rhizogenes 5-3-2 strain

[0014] The present invention provides a strain that uses pathogenic antagonistic microorganisms to control tomato bacterial wilt, specifically Rhizobium rhizogenes 5-3-2. In 2008, the experimenters collected continuous cropping vegetables from different regions such as Jinhua, Taizhou and Lishui. In soil samples from the ground, more than 300 strains of various types of bacteria were isolated from the soil samples using the bacterial culture medium by dilution and separation method, and the strains were purified, and then the strains were cultured at 28-30°C through slants and shake flasks. The inoculation effect of various seedling substrates was determined, and the strain 5-3-2 that antagonized tomato bacterial wilt was screened out.

[0015] 1. Medium

[0016] Beef extract peptone agar medium: beef extract 3g, peptone 10g, NaCl 5g, agar 15-20g, H 2 O 1000ml, pH 7.0-7.2. Sterilize at 121.3°C for 20 minutes.

[0017]...

Example Embodiment

[0034] Example 2: Plate antagonism test of the isolated bacteria against Ralstonia solanacearum

[0035] 1. Medium

[0036] 1.1 Solid medium

[0037] Ralstonia solanacearum culture medium preparation: MgSO 4 7H 2 O0.3g, K 2 HPO 4 2.0g, Yeast extract 4.0g, Caseinhydrolysate 8.0g, Sucrose 10.0g, Agar 18g, Distilled water 1000ml.

[0038] Preparation of beef extract peptone agar medium: same as in Example 1.

[0039] 1.2 Liquid medium

[0040] Bacterial liquid shake flask culture medium: Beef extract peptone culture medium does not add agar.

[0041] Tomato Ralstonia solanacearum liquid shake flask culture medium: Ralstonia solanacearum culture medium does not add agar.

[0042] 2. Test procedure

[0043] 2.1 Medium preparation

[0044] Preparation of Ralstonia solanacearum culture medium (solid, liquid), preparation of pathogenic bacterium Ralstonia solanacearum slant; preparation of beef extract peptone agar medium (solid, liquid), preparation of bacterial slant.

[0045] 2.2 Plate Antagonis...

Example Embodiment

[0087] Example 3: Preparation of antagonist of strain 5-3-2

[0088] The microbial agent capable of antagonizing tomato bacterial wilt of the present invention is cultured and multiplied by Rhizobium rhizogenes 5-3-2 strain.

[0089] The specific operation process is as follows: Rhizobium rhizobium 5-3-2 is based on beef extract medium, adding a small amount of inorganic salts such as calcium, magnesium, phosphorus, etc. to carry out liquid shake flask fermentation, culture at 25-35°C, rotating speed 120-220rpm. Use this as a strain to inoculate a liquid fermentor to make a liquid mother seed. The raw materials of the liquid fermentor are bran, vegetable cake, and bean cake powder (ratio 1%~5%:0~3%:0~3%:0~3%) ) And a small amount of inorganic salts, etc., the amount of water added is 99-86%. After autoclaving, when the temperature of the fermenter liquid is cooled to below 35℃, then 1.0-10% of the inoculum will be inoculated into the liquid fermentation by aseptic operation In the...

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Abstract

The invention provides a strain for preventing and controlling tomato bacterial wilt by using pathogenic bacteria antagonistic microbe, namely rhizobiumrhizogenes 5-3-2, wherein the preservation number of the rhizobiumrhizogenes 5-3-2 is CGMCC No.3333. The invention also provides a bactericide prepared from the rhizobiumrhizogenes 5-3-2. The invention also provides application of the rhizobiumrhizogenes 5-3-2 in preventing and controlling the tomato bacterial wilt, in particular application of the rhizobiumrhizogenes 5-3-2 serving as seedling raising matrix in preventing and controlling the tomato bacterial wilt. The rhizobiumrhizogenes 5-3-2 is directionally separated and selected from the nature, a bactericide product is prepared by culturing and propagating the rhizobiumrhizogenes 5-3-2, the seedling raising matrix is prepared by fermenting and rotting mushroom residue after the edible mushroom is cultured and blending the mushroom residue and other base materials in a certain ratio, and the biological control bactericide and the seedling raising matrix are organically combined, so the application has the advantages of rich available resource, low cost and capability of avoiding secondary pollution of the environment and is an effective way for solving the soil-borne disease-tomato bacterial wilt.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a tomato bacterial wilt antagonistic strain and the field of biological control of tomato bacterial wilt by a seedling-raising substrate prepared by using the bacterial agent of the bacterial strain. Background technique [0002] Mushroom dregs are mainly discarded mushroom tubes after cultivation such as shiitake mushrooms and enoki mushrooms. The main components are wood dregs and cottonseed shells, which are mainly lignin and have low nutrient content. After being decomposed by edible fungi, the mushroom dregs are slightly acidic and soft , Its water absorption, water retention and fertility, and air permeability are good, and it is more suitable for the growth of seedling roots of flower seedlings. Therefore, mushroom dregs are more suitable for developing container seedling production substrates for flower seedlings. Therefore, the research and development g...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C05F11/08C12R1/41
Inventor 王卫平薛智勇朱凤香陈晓旸洪春来吴传珍
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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