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Methods for the directed expansion of epitopes for use as antibody ligands

An antibody and epitope technology, which can be used in the preparation methods of peptides, pharmaceutical formulations, antibody medical components, etc., and can solve problems such as logistics and cost obstacles and limitations

Inactive Publication Date: 2010-09-29
PEPTIMMUNE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of antigens purified from raw materials is limited by logistical and cost barriers

Method used

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  • Methods for the directed expansion of epitopes for use as antibody ligands
  • Methods for the directed expansion of epitopes for use as antibody ligands
  • Methods for the directed expansion of epitopes for use as antibody ligands

Examples

Experimental program
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Effect test

Embodiment 1

[0135] Example 1 Preparation of DSP Compositions Based on Fictitious Peptides

[0136] For ease of understanding, and by way of example, the table in Figure 6 describes and represents the preparation of DSP compositions from two imaginary peptide sequences representing a known epitope. In this example, each box consists of 5 amino acids, (y 1 In x1, x2, x3, x4, x5=THMCE, and y 2 in PWKNA). The THMCE is defined to have input ratios of a=7, b=1, c=1, d=1, e=10. PWKNA is defined to have input ratios a=1, b=3, c=3, d=3, e=20. For synthesis, the group of amino acids occupying each amino acid position of each peptide is identified by the preferred amino acid substitution method shown in Figure 4 and described in Kosiol et al., J. Theoretical Biol. 228:97-106 , 2004 (or an equivalent method that systematically changes amino acids is less preferred), and the total ratio of amino acids occupying each position in the resulting overall DSP composition is given above. Each box, yi an...

Embodiment 2

[0140] Example 2 Preparation of Gp100 (amino acid residues 154-162) as the DSP composition of the source peptide

[0141] Figures 7A-B show examples of DSP synthesis rules using Gp100 (amino acid residues 154-162) as the source peptide. The method and rules for defining the amino acid species at each position of the generated peptides are described in Example 1 above. As in Example 1, the DSP composition was synthesized by a solid-phase peptide synthesis method.

Embodiment 3

[0142] Embodiment 3 prepares the DSP composition of HLA peptide as source peptide

[0143] Figures 8A-B show examples of DSP synthesis rules using HLA-derived peptides and HLA-mimetic source peptides as source peptides. The method and rules for defining the amino acid species at each position of the generated peptides are described in Example 1 above. As in Example 1, the DSP composition was synthesized by a solid-phase peptide synthesis method.

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Abstract

The instant invention comprises a process for selecting and manufacturing antibodies useful for therapeutic, prophylactic, diagnostic or research purposes using epitope peptide mixtures synthesized by the solid phase synthesis, such process defined by a set of rules regarding the identity and the frequency of occurrence of amino acids that substitute a base or native amino acid of a known epitope. The resulting antibodies are related to but distinct from antibodies that bind to the known epitope.

Description

[0001] Related patents [0002] This application claims U.S. Provisional Application 60 / 928,225, filed May 7, 2007, U.S. Provisional Application 60 / 999,283, filed October 16, 2007, U.S. Provisional Application 60 / 999,284, filed October 16, 2007 and priority of US provisional application 61 / 124,689, filed April 17, 2008. This application is also a continuation-in-part of US Application 11 / 787,229, filed April 13, 2007, which claims priority to US Provisional Application 60 / 792,085, filed April 13, 2006. The contents of all applications listed above are incorporated herein by reference in their entirety. Background of the invention [0003] In recent years, antibodies have solidified their status as an effective class of therapeutic drugs for a variety of diseases and conditions. At present, 21 kinds of antibody-based drugs have been approved in the world, and a large number are in the process of being completed. [0004] A single antibody may be specific for one antigen, or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/145C07K1/04
CPCC07K1/047C07K16/00A61K39/145C07K1/04Y02A50/30
Inventor D·伯恩
Owner PEPTIMMUNE
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