Quantitative optical molecular tomographic device and reconstruction method

Abstract

The invention discloses a quantitative optical molecular tomographic device and a quantitative reconstruction method. The molecular tomographic device comprises a bioluminenscent tomographic data acquisition platform, a Micro CT (Computerized Tomography) system, a quantitative calibration system and a quantitative reconstruction computer, wherein the bioluminenscent tomographic data acquisition platform is used for capturing the distribution condition of bioluminescent light sources emerging on the body surface of a small animal, and the Micro CT system is used for acquiring the anatomical structure information of the small animal body. The quantitative reconstruction method comprises a quantitative method of a receiving light source based on a field of view and a reconstruction method based on a finite element subdivision network. By utilizing the quantitative optical molecular tomographic device and the quantitative reconstruction method, three-dimensional light source distribution and the quantitative information of a cell number in a small animal body can be inverted through the two-dimensional light source distribution and the quantitative energy information on the surface of the small animal body.

Description

technical field [0001] The invention relates to the technical field of optical imaging, in particular to the construction of a quantitative molecular imaging system and a quantitative reconstruction method of a light source. Background technique [0002] In the past ten years, the technology of bioluminescence imaging (BLI) has developed rapidly, and has been widely used in biological research, and has achieved remarkable scientific research results. However, bioluminescent imaging technology can only generate two-dimensional plane images, and cannot intuitively display the three-dimensional spatial position information of the light source in the small animal body, which has great limitations in the visualization effect. In terms of quantitative analysis, bioluminescent imaging can only perform relative quantitative analysis, but does not have absolute quantitative significance, while the three-dimensional imaging technology of tomography has absolute quantitative significan...

AI Technical Summary

Problems solved by technology

However, these two regularization terms are the only ones selected during the reconstruction process, and are not adjusted according to the feasible area of ​​the reconstructed light source, which still has certain limitations in accurately describing the nature of the light source.

Iterative or optimization methods have been widely used to reconstruct light sources. The current algorithms are based on the nodes or discrete units in the numerical discrete method to search. This search method is extremely flexible, but it is also very sensitive to noise.

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