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Liquid fermentation method capable of improving yield of ganoderma iucidum polysaccharide

A technology of liquid fermentation and Ganoderma lucidum polysaccharide is applied in the field of fermentation engineering, which can solve the problems of long fermentation period and low production efficiency, and achieve the effects of high production efficiency, short period and wide source.

Inactive Publication Date: 2012-09-05
INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of this method is that static culture is required to induce the synthesis of products, and the fermentation period is long. The total fermentation time of liquid aerobic fermentation plus static culture needs more than 20 days, while the fermentation time of general ganoderma products is 5 to 8 days. , so its overall production efficiency is low

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  • Liquid fermentation method capable of improving yield of ganoderma iucidum polysaccharide
  • Liquid fermentation method capable of improving yield of ganoderma iucidum polysaccharide

Examples

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Effect test

Embodiment 1

[0041] The ganoderma strain is Taishan red ganoderma lucidum (Ganoderma lucidum), and the strain number is CGMCC No.5.644.

[0042] A method for improving the liquid fermentation of Ganoderma lucidum polysaccharide by using rare earth elements, the specific steps are:

[0043] 1. Preparation of rare earth element mother liquor

[0044]Weigh praseodymium nitrate, neodymium nitrate and lanthanum nitrate with a purity of 99%, respectively, and prepare solutions with a concentration of 1 mol / L with distilled water. It can be used as the mother liquor of rare earth elements.

[0045] 2. Preparation of Ganoderma lucidum seed solution

[0046] Inoculate Ganoderma lucidum strain CGMCC No.5.644 into PD liquid fermentation medium (200g of potatoes, 20g of glucose, 1000mL of distilled water) for fermentation and cultivation. One day, get the Ganoderma lucidum seed liquid.

[0047] 3. Take 3 parts of each of the 3 kinds of rare earth element mother solutions prepared in step 1, and ad...

Embodiment 2

[0056] The ganoderma strain is Taishan red ganoderma lucidum (Ganoderma lucidum), and the strain number is CGMCC No.5.644.

[0057] A method for improving the liquid fermentation of Ganoderma lucidum polysaccharide by using rare earth elements, the specific steps are:

[0058] 1. Preparation of rare earth element mother liquor

[0059] Weigh praseodymium nitrate, neodymium nitrate and lanthanum nitrate with a purity of 99%, respectively, and prepare solutions with a concentration of 1 mol / L with distilled water. It can be used as the mother liquor of rare earth elements.

[0060] 2. Preparation of Ganoderma lucidum seed solution

[0061] Inoculate Ganoderma lucidum strain CGMCC No.5.644 into PD liquid fermentation medium (potato 200g, glucose 20g, distilled water 1000mL) for fermentation and culture. One day, get the Ganoderma lucidum seed liquid.

[0062] 3. Take 3 parts of each of the 3 kinds of rare earth element mother solutions prepared in step 1, and add them to the ...

Embodiment 3

[0070] Embodiment 3: as described in Example 1, the difference is that the ganoderma lucidum strain is Jingda Ganoderma lucidum (Ganoderma lucidum) strain number is CGMCC No.5.533; the rare earth element described in step 3 is praseodymium Pr 3+ , the result that the exopolysaccharide production of Ganoderma lucidum can reach 435.22mg / L.

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Abstract

The invention relates to a liquid fermentation method capable of improving the yield of ganoderma iucidum polysaccharide. In the method, ganoderma lucidum cells are cultured in liquid, and a rare earth element praseodymium, neodymium or lanthanum is added into a culture medium to improve the yield of the ganoderma iucidum polysaccharide. The ganoderma iucidum polysaccharide can be used in preparing medicaments for regulating immunity, resisting cancers, reducing blood sugar and the like.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a liquid fermentation method for increasing the yield of ganoderma lucidum polysaccharide by using rare earth elements. Background technique [0002] Ganoderma lucidum belongs to Basidiomycetes, Polyporaceae, Ganoderma fungus. In ancient China, it was widely known as the fairy grass. Ancient medical scientists discovered that Ganoderma lucidum can keep the human body in a state of balance by strengthening the body. Ganoderma lucidum polysaccharide is the most important physiologically active substance of Ganoderma lucidum. Modern pharmacological studies have shown that Ganoderma lucidum has anti-tumor, anti-aging, and anti-arteriosclerosis effects. While the medicinal value of Ganoderma lucidum has been widely revealed, the yield of Ganoderma lucidum polysaccharides has become a limiting factor inhibiting its application, because wild Ganoderma lucidum is very r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 姚强高兴喜宫志远任鹏飞任海霞韩建东刘岩李瑾曲玲赵军胜
Owner INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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