Method for detecting clenbuterol through combination of MIT technology and SPR technology

A combination of technology and technology, applied in the field of clenbuterol detection, can solve the problems of poor selectivity, high cost, harsh detection environment, etc., and achieve the effect of short reaction time, easy operation, and not easy to biodegrade and destroy

Inactive Publication Date: 2010-11-10
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention aims to solve the problems of harsh detection environment, high cost and poor selectivity in the detection of cl

Method used

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  • Method for detecting clenbuterol through combination of MIT technology and SPR technology
  • Method for detecting clenbuterol through combination of MIT technology and SPR technology
  • Method for detecting clenbuterol through combination of MIT technology and SPR technology

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Experimental program
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Embodiment

[0040] (1) First, a 47nm-thick gold film was deposited on the optical cover glass by vacuum evaporation method, which was used as the gold film substrate.

[0041] (2) Dissolve the purified clenbuterol in acetonitrile to make a 0.05mol / L solution, draw 0.36mL of the solution into a vial, then add acetonitrile to make a 0.02mol / L solution. Then add 5 μL of methacrylic acid, after ultrasonication for 10 min, let stand for 3 h.

[0042] (3) Add 27.5 μL of cross-linking agent ethylene glycol dimethacrylate and 0.80 mg of initiator azobisisobutyronitrile to the above-mentioned vial containing the mixed solution, sonicate for 10 minutes, and deoxygenate with nitrogen gas for 5 minutes;

[0043] (4) Rinse the silanized glass slide with ethanol, and dry it with nitrogen gas, then stick the sealing film tightly on the surface of the silanized glass slide, and then process a blank area in the middle area of ​​the sealing film as a film-forming area, Obtain a silanized glass slide with ...

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Abstract

The invention relates to a method for detecting clenbuterol through the combination of a MIT technology and a SPR technology, belonging to the field of clenbuterol detection. The method of the invention comprises: firstly using a thermal polymerization method to prepare a clenbuterol molecular imprinting polymer film on a gold film substrate, removing template molecules with eluent, and obtaining a clenbuterol molecular imprinting sensor chip; then calculating the displacement value Delta Theta of SPR resonance angle resulting from the post-reaction and the pre-adsorption of the clenbuterol standard solution and the sensor chip, and creating a standard curve of negative logarithm of Delta Theta and the concentration value of the clenbuterol standard solution; and finally according to the displacement value Delta Theta of the SPR resonance angle resulting from the post-reaction and the pre-adsorption of the clenbuterol standard solution and the sensor chip, and reading the corresponding clenbuterol content on the standard curve. The method of the invention has the characteristics of severe-environment-resistance, high selectivity, fast response and simple operation, not only improves the sensitivity and limit of detection of the method, but also correspondingly reduces the detection cost, and also almost has no adsorption to salbutamol having similar structure with clenbuterol.

Description

technical field [0001] The invention relates to a method for detecting clenbuterol by combining MIT technology and SPR technology, and belongs to the field of clenbuterol detection. Background technique [0002] Clenbuterol (CL), also known as clenbuterol, is a β-adrenergic agonist (abbreviated as β-stimulant), which is commonly used in medicine to treat asthma. It can promote animal growth, reduce fat content, and increase lean meat rate, so it is often used as a feed additive, commonly known as clenbuterol. It will form residues in animal tissues, and humans will cause poisoning after eating pork containing clenbuterol, usually with symptoms such as muscle tremors, heart palpitations, nervousness, nausea, and dizziness. The Chinese government banned clenbuterol hydrochloride as a feed additive in 1997. However, in recent years, clenbuterol hydrochloride poisoning due to consumption of commercially available pigs, poultry meat and internal organs has occurred frequently, ...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/41
Inventor 韦天新孟慧娟
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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