Multiplex PCR detection method of wheat yellow dwarf virus

Abstract

The invention relates to a multiplex PCR detection method of wheat yellow dwarf virus, comprising the following steps: extracting total RNA of wheat virus, preparing a cDNA template by reverse transcription reaction, performing multiplex PCR reaction, performing electrophoresis detection and determining the type of virus, wherein in the step of preparing a cDNA template by the reverse transcription reaction, primers shown in SEQ ID NO.2, SEQ IDNO.4 and SEQ ID NO.6 are utilized; and in the step of performing multiplex PCR reaction, the primers shown in SEQ ID NO.1-6 are utilized. When being used to detect complex infection wheat materials in fields, the multiplex PCR detection method of wheat yellow dwarf virus in the invention has the advantage of being rapid, saving time, saving cost and the like.

Description

technical field [0001] The invention belongs to the technical field of plant virology, in particular to a multiple PCR detection method for wheat yellow dwarf virus. Background technique [0002] Wheat is one of the most important food crops in the world, and my country's planting area ranks first in the world. However, viral diseases and other diseases have seriously affected the yield and quality of wheat. Barley yellow dwarf virus (Barley yellow dwarf virus, BYDV) is a global viral disease. Every year, it causes a certain degree of harm to the world's grain production, and it will cause serious reduction in production when it occurs. In my country, the disease is mainly prevalent in northern wheat regions, and the wheat yellow dwarf caused by it is an important epidemic disease. In 1987, only two provinces of Shaanxi and Gansu lost more than 500 million kilograms of wheat due to the epidemic of the disease. In 1998, the large-scale epidemic occurred in many provinces (...

AI Technical Summary

Problems solved by technology

[0003] Early detection methods: biology, serology, electron microscope observation, nucleic acid hybridization and PCR technology have been used to detect wheat viruses, but some traditional detection methods are not very sensitive, and they are only for the detection of a single virus. It takes a lot of time and reagents to detect mixed viruses

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