Methods and compositions for immunizing pigs against porcine circovirus

A technology of porcine circovirus and composition, applied in the direction of antiviral agent, virus, drug combination, etc., can solve the problem of specific protein paradigm without immunogenic properties and so on

Inactive Publication Date: 2010-12-29
WYETH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Proteins encoded by the PCV2 open reading frame (ORF) consisting of ORF1-ORF13 have been extensive...

Method used

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  • Methods and compositions for immunizing pigs against porcine circovirus
  • Methods and compositions for immunizing pigs against porcine circovirus
  • Methods and compositions for immunizing pigs against porcine circovirus

Examples

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preparation example Construction

[0131] Subunit vaccines are generally prepared differently than modified live or inactivated vaccines. Before subunit vaccines can be prepared, the protective or antigenic components of the vaccine must be identified. These protective or antigenic components include specific amino acid segments or fragments of viral capsid proteins, which elicit particularly strong protective or immunogenic responses in pigs; single or multiple viral capsid proteins themselves, which oligomeric bodies, and higher-level associations of viral capsid proteins that form viral substructures or identifiable parts or units of these substructures; oligoglycosides, glycolipids present on or near the viral surface or within viral substructures Or glycoproteins, such as lipoproteins or lipid groups associated with viruses, and so on. Preferably, a capsid protein, such as the protein encoded by the ORF2 gene, is used as an antigenic component of a subunit vaccine. Other proteins encoded by infectious DN...

Embodiment 1

[0161] Isolation and identification of two novel porcine circovirus type 2B strains

[0162] A study was planned to test a novel vaccine formulation in pigs to assess its efficacy against porcine circovirus and Chlamydia hyopneumoniae. During the course of the study, several pigs in the control and vaccinated groups were observed to show symptoms of PMWS. Environmental exposure of these pigs to PCV2 prior to challenge was then confirmed. Molecular analysis of blood and tissue samples from these pigs revealed that they had a type 2B strain that was different from the strain used for the challenge. Furthermore, sequence analysis established that the PCV2B strain annotated FD07 and another novel PCV2B pig (annotated FDJE) isolated from pigs on the farm were distinct from other type 2B strains identified in other field studies and previously identified by others. Materials and methods used to isolate and characterize these two novel PCV2B strains are described below.

[0163] M...

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Abstract

The present invention relates to the isolation and identification of two new strains of type 2B porcine circovirus. These two new strains of porcine circovirus may be used for the preparation of vaccine or immunogenic compositions for immunizing pigs against postweaning multisystemic wasting syndrome (PMWS). Accordingly, the invention provides methods for eliciting a protective immune response against a pathogenic porcine circovirus by administering to a pig an immunogenically effective amount of a type 2B porcine circovirus vaccine or immunogenic composition comprising at least one of the porcine circoviruses having a nucleic acid sequence as set forth in SEQ ID NOs: 1 or 2, or at least one protein from at least one of the two new type 2B strains of porcine circovirus as described herein. The invention further relates to protection of a pig from any one or more of the symptoms or sequelae associated with PMWS.

Description

field of invention [0001] The present invention relates to the field of animal health and provides methods and compositions for protecting pigs against pathogenic porcine circovirus type 2B strains. More specifically, the present invention relates to newly identified pathogenic porcine circovirus type 2B strains, nucleic acid sequences encoding these type 2B strains and proteins encoded by these nucleic acid sequences. The present invention also relates to methods and compositions for inducing an immune response to pathogenic porcine circoviruses by administering at least one of these type 2B porcine circoviruses comprising an immunogenic effective amount, or encoding these 2B A composition of at least one nucleic acid of at least one of porcine circoviruses or at least one of the proteins encoded by these nucleic acids. Background of the invention [0002] Porcine circovirus (PCV) is a small icosahedral non-enveloped virus with a single-stranded circular DNA genome of appr...

Claims

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Application Information

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IPC IPC(8): C12N7/00
CPCC12N2750/10021C12N7/00C12N2750/10034A61P31/12A61P31/20A61P37/04
Inventor S·Q·吴
Owner WYETH LLC
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