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Dimethylformamide degrading bacteria and bacterial agent produced from same

A technology of dimethylformamide and degrading bacteria, which is applied in the field of biotechnology, can solve problems such as environmental pollution, and achieve the effects of reducing costs, protecting the ecological environment, and being easy to use

Active Publication Date: 2011-12-21
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the wastewater discharged from the production process of the above chemical products contains a large amount of DMF, and only about 100 million tons of DMF-containing wastewater is discharged from the leather industry every year, causing serious pollution to the environment.

Method used

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  • Dimethylformamide degrading bacteria and bacterial agent produced from same
  • Dimethylformamide degrading bacteria and bacterial agent produced from same
  • Dimethylformamide degrading bacteria and bacterial agent produced from same

Examples

Experimental program
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Embodiment Construction

[0020] 1 Laboratory biodegradation experiment

[0021] 1.1 Effect of inoculum size on DMF degradation efficiency

[0022] Strain DMF3 was cultured in LB medium (LB medium formula g / L: yeast extract 5.00, peptone 10.00, NaCl 10.00, pH 7.0) to the late logarithmic period, and inoculated at 0.5, 1, 2, 5, 10, 15% The amount was added to the inorganic salt medium (DMF 400mg / L, NaCl 1.00g / L, K 2 HPO 4 2.50g / L, KH 2 PO 4 1.00g / L, pH 7.0. hereinafter the same), cultured on a shaker at 30°C and 160r / min, and measured the content of DMF every 12 hours, and the results were as follows: image 3 shown. The size of the inoculum size has a direct relationship to the degradation efficiency of DMF. Under the inoculum size less than 2%, the larger the inoculum size, the higher the degradation efficiency of DMF; DMF degradation has little effect.

[0023] 1.2 Effect of initial pH on DMF degradation efficiency

[0024] Bacterial strain DMF3 was cultivated in LB medium to late logarith...

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Abstract

The invention provides dimethylformamide (DMF) degrading bacteria and a bacterial agent produced from the same, which belong to the field of biological high technology. The strain is a Gram staining reaction negative bacteria strain DMF3 and is identified as pseudomonasplecoglossicida. The strain has the main biological characteristic of G<->, an irregular rod-shaped strain body, the size of about 0.5-1.0*1.5-5.0 mu m and no stipe or mycoclena, does not produce spore, is aerobic, undergoes strict respiratory metabolism and can grow by taking DMF as a unique carbon source and nitrogen source so as to be completely mineralized for releasing ammonia nitrogen. The strain can reduce the residual quantity of the DMF with certain concentration in waste water by over 95 percent at the temperature of 30 DEG C under the pH value of 7.0 within 3 days, so that the problem of difficult treatment of the DMF in chemical production waste water is solved, the chemical oxygen demand (COD) of the DMF waste water reaches the standard and the waste water can be discharged.

Description

1. Technical field [0001] The invention provides a dimethylformamide-degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology and are used to degrade dimethylformamide, a commonly used chemical solvent, for waste water treatment by means of microorganisms. 2. Technical background [0002] Dimethylformamide (DMF) is an organic solvent with stable chemical properties, high boiling point and excellent performance. It has a density of 0.94, a boiling point of 152.8°C and a freezing point of -61°C. Except for halogenated hydrocarbons, it can be miscible with water and most organic solvents in any ratio. Because of its good solubility, it is called "universal solvent". And DMF is an important pesticide and pharmaceutical intermediate, an absorbent for various gases: in pesticides, it is used to synthesize dimeform; in medicine, it is used to synthesize sulfadiazine, doxycycline, cortisone and vitamins Drugs such as B6; in petroc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/38
Inventor 蔡天明陈立伟杨帅顾海莎
Owner NANJING AGRICULTURAL UNIVERSITY
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