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Kit for detecting CEBPA gene mutation

A kit and gene technology, applied in the field of kits for detecting CEBPA gene mutations, to achieve the effect of perfecting the genetic diagnosis system

Inactive Publication Date: 2011-01-19
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these studies are still in the preliminary stage in our country, and only some molecular markers are used in clinical examination, and there are still many molecular markers that are beneficial to patient diagnosis, prognosis assessment and treatment, such as CEBPA, MLL-PTD, FLT3-TKD, etc., which urgently need to be developed.

Method used

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  • Kit for detecting CEBPA gene mutation
  • Kit for detecting CEBPA gene mutation
  • Kit for detecting CEBPA gene mutation

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Experimental program
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Effect test

Embodiment

[0037] 1. Preparation of kits

[0038] 1. Synthesis of 4 pairs of fluorescently labeled primers

[0039] First pair: 1F: 5'-FAM-GGCGAGCAGGGTCTCCGGGT-3' (SEQ ID NO: 1);

[0040] 1R: 5'-TGTGCTGGAACAGGTCGGCCA-3' (SEQ ID NO: 2);

[0041] Second pair: 2F: 5'-HEX-GCTGGGCGGCATCTGCGA-3' (sequence 3);

[0042] 2R: 5'-CCCCGACGCGCTCGTACAGG-3' (SEQ ID NO: 4);

[0043] The third pair: 3F: 5'-FAM-CCGGCTACCTGGACGGCAGG-3' (SEQ ID NO: 5);

[0044] 3R: 5'-CGTTGCTGTTCTTGTCCACCGACTTCTT-3' (SEQ ID NO: 6);

[0045] The fourth pair: 4F: 5'-HEX-CTCGGTGCCGCCGGCCT--3' (SEQ ID NO: 7);

[0046] 4R: 5'-AACCACTCCCTGGGTCCCCGC-3' (SEQ ID NO: 8);

[0047] 2. Synthesis of 4 pairs of primers

[0048] Synthesize four pairs of primers that do not carry labeling groups, and the primer sequences are the same as step 1.

[0049] Using the DNA of the subject to be tested as a template and using the above 4 pairs of primers to amplify, 4 kinds of amplification products can be ob...

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Abstract

The invention discloses a kit for detecting CEBPA gene mutation. The invention discloses a special primer composed of primer pairs 1, 2, 3 and 4; primer 1 is composed of DNAs shown as sequence 1 and sequence 2 in a sequence list; the primer pair 2 is composed of DNAs shown as sequence 3 and sequence 4 in the sequence list; primer pair 3 is composed of DNAs shown as sequence 5 and sequence 6 in the sequence list; and primer pair 4 is composed of DNAs shown as sequence 7 and sequence 8 in the sequence list. The invention also provides a special primer containing kit for detecting CEBPA gene mutation. The invention has clinic significance for conventional screening CEBPA gene mutation on AML patients, contributes to further knowing of molecule characteristic of AML patients in China and perfection on gene diagnosis system, is used for more accurate molecular classification, individuation diagnosis and prognosis prediction, thus providing the foundation for selecting appropriate treatment method for patients and having significance on clinic AML diagnosis, layering and prognosis evaluation.

Description

technical field [0001] The invention relates to a kit for detecting CEBPA gene mutation. Background technique [0002] 1. Research overview of molecular markers of acute myeloid leukemia [0003] Acute myeloid leukemia (AML) is a malignant disease in which the cumulative acquired gene changes of myeloid hematopoietic stem / progenitor cells lead to changes in normal cell proliferation, differentiation and apoptosis pathways. The main types of the disease are complicated in classification, the pathogenesis is still unclear, and they have significant heterogeneity both clinically and genetically. Cytogenetic abnormalities can be identified in approximately 55% of adult AML patients, and approximately 45% of AML patients exhibit a normal karyotype. Recently, a variety of molecular markers with important clinical prognostic value have been identified in AML with normal karyotype. Risk stratification by molecular markers plays an increasingly important role in diagnosis and treat...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 阮国瑞黄晓军陈珊珊刘艳荣李金兰牛继红李玲娣秦亚溱
Owner PEOPLES HOSPITAL PEKING UNIV
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