Swine promoter protein expression vector and construction method and application thereof

Abstract

The invention discloses a swine promoter protein expression vector and a construction method and application thereof. The expression vector is annular and comprises a pronucleus replication origin, an eukaryon replication origin, a selection marker gene and an exogenous gene expression cassette, wherein the exogenous gene expression cassette consists of a transcription promoter, a junction fragment and a polyadenylic acid tailing signal sequentially from upstream to downstream; and the transcription promoter is an MLP6 promoter. The expression vector can efficiently express exogenous proteinsin a swine cell, contains the eukaryon replication origin and can be proliferated greatly in a cell of an expression T antigen, so that protein expression level is raised. A recombinant vector of an expression exogenous gene is constructed by homologous recombination, so that the conventional steps such as enzyme cutting, linking and the like are avoided and 2 to 3 steps are saved compared with the conventional linking process. A vector recombination process for constructing the expression exogenous gene by using the vector of the invention is simple, convenient, rapid, economical and efficient and the construction of the expression vectors of a large number of exogenous genes can be finished rapidly.

Description

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AI Technical Summary

Benefits of technology

People have been trying for better ways to make viruses that infect animal tissues more effective at producing therapeutic agents or vaccines against diseases caused by virus strain(s). However, current methods require complicated processes like cloning and transformation from yeast to mammals, making it difficult to create multiple types of immunogenic compositions simultaneously without introducing new ones during production.

Problems solved by technology

People have long sought ways for better genetic engineering techniques that could lead to more efficient growth of crops like chickens (chicken). However, current approaches require expensive equipment and complicated procedures involving multiple steps, making them slow down during constructional processes. Therefore there remains room for improvement over existing technologies without compromising their effectiveness at producing desired traits from these plants.

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