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Enzymatic method for extracting soy peptide

A technology of enzymatic extraction and soybean peptide, which is applied in the field of enzymatic extraction of soybean protein peptide and soybean peptide, can solve the problems of destroying protein components, not using membrane separation technology, and reducing the economic benefits of enterprises, so as to achieve short production cycle, Guaranteed natural whiteness and low cost

Inactive Publication Date: 2012-07-25
湖北远成赛创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A large amount of alkali and organic solvents are used for treatment, which not only brings serious environmental pollution and potential safety hazards, but also destroys the protein components; moreover, no membrane separation technology is used in the prior art, which reduces the economic benefits of the enterprise

Method used

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  • Enzymatic method for extracting soy peptide
  • Enzymatic method for extracting soy peptide
  • Enzymatic method for extracting soy peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for enzymatically extracting soybean peptides, the steps are as follows:

[0024] Weigh 1 kg of commercially available soybean protein powder (protein ≥ 55%, moisture ≤ 7%, fat ≤ 1%, ash ≤ 4%, total fiber ≤ 4%), add 7 kg of distilled water, 55 g of 95 wt% ethanol and 15 g of anhydrous Sodium sulfite, after stirring evenly, heat up to 90°C for reflux cooking for 5 hours, then filter while hot; then cool the filtrate to 50°C, then add 20g of alkaline protease, and adjust the pH to 7- 8. Stir the enzymolysis at 50°C for 6 hours. During the enzymolysis process, use the above sodium hydroxide solution to keep the pH value of the reaction system stable; then add 15g of flavor protease, and use the above 0.1M sodium hydroxide solution to keep the pH value of the reaction system at 7. -8, enzymolysis at 50°C for 8 hours; then heat up to 90°C, keep warm for 14 minutes to inactivate the enzyme; then filter the enzymolysis solution to obtain a supernatant; cool the supern...

Embodiment 2

[0029] A method for enzymatically extracting soybean peptides, the steps are as follows:

[0030] Weigh commercially available soybean protein powder (protein ≥ 55%, moisture ≤ 7%, fat ≤ 1%, ash ≤ 4%, total fiber ≤ 4%) 2kg, add 15kg distilled water, 100g 95wt% ethanol and 30g anhydrous Sodium sulfite, after stirring evenly, heat up to 92°C for reflux cooking for 4 hours, then filter while hot; then cool the filtrate to 55°C, then add 60g of alkaline protease, adjust the pH to 7- 8. Stir the enzymolysis at 55°C for 5 hours. During the enzymolysis process, use the above-mentioned sodium hydroxide solution to keep the pH value of the reaction system stable; then add 30g of flavor protease, and use the above-mentioned 0.1M sodium hydroxide solution to keep the pH value of the reaction system at 7- 8. Enzymolysis at 55°C for 7 hours; then heat up to 95°C and keep warm for 16 minutes to inactivate the enzyme; then filter the enzymolysis solution to obtain a supernatant; cool the sup...

Embodiment 3

[0035] A method for enzymatically extracting soybean peptides, the steps are as follows:

[0036] Take commercially available soybean protein powder (protein ≥ 55%, moisture ≤ 7%, fat ≤ 1%, ash ≤ 4%, total fiber ≤ 4%) 10kg, add 80kg of distilled water, 600g of 95wt% ethanol and 200g of Sodium sulfite in water, stir evenly, heat up to 93°C for reflux cooking for 4 hours, then filter while hot; then cool the filtrate to 55°C, then add 400g of alkaline protease, adjust the pH to 7 with 0.1M food-grade sodium hydroxide solution Stir at -8, 55°C for 5 hours, use the above sodium hydroxide solution to keep the pH value of the reaction system stable during the enzymolysis process; then add 200g of flavor protease, and use the above 0.1M sodium hydroxide solution to keep the pH value of the reaction system at 7 Enzymolysis at -8, 55°C for 6 hours; then heat up to 96°C, keep warm for 20 minutes to inactivate the enzyme; then filter the enzymolysis solution to obtain a supernatant; cool...

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Abstract

The invention discloses an enzymatic method for extracting a soy peptide. In the method, the soy peptide is obtained by using soybean protein powder as a main raw material through the steps of: distributing water, adding alcohol and sodium sulfite, heating, boiling, filtering, adding protease into a filtrate for enzymolysis, inactivating enzyme, decoloring, separating membrane, and drying the obtained filtrate. The method has the advantages of short production cycle, low cost and no generation of toxic and harmful substances, and can be widely applied to the fields of foods, medicines, healthcare products, biosynthesis, and the like; and the obtained products has high safety.

Description

technical field [0001] The method of the present invention relates to the technical field of soybean protein deep processing, in particular to a method for enzymatically extracting soybean protein peptides, which uses commercially available soybean protein powder as a raw material, and utilizes biological enzyme enzymatic hydrolysis and biological enzyme modification technology to extract protein peptides. A method for modifying and extracting soybean peptides. Background technique [0002] Soybean protein is the protein contained in soybean products, with a content of about 38%, which is 4 to 5 times that of cereal foods. The amino acid composition of soybean protein is similar to that of milk protein. Except for slightly lower methionine, the content of other essential amino acids is relatively rich. It is a complete vegetable protein and can be equivalent to animal protein in terms of nutritional value. FAO / WHO (1985) human test results show that the essential amino acid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/12
Inventor 叶传发张小东胡承四叶思
Owner 湖北远成赛创科技有限公司
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