Culture medium for producing trichodermin by trichoderma harziarum strain L through fermentation

A technology of culture medium and trichocin, which is applied in the field of microorganisms, can solve the problems of low production capacity and low level of hormone production, and achieve the reliable effect of improving the level of hormone production.

Inactive Publication Date: 2011-02-23
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wild-type strains isolated from nature often have l

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] Cultivation of Fusarium wilt of cucumber: purchased from Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences. The culture medium is potato dextrose liquid medium (PD), 60 mL of PD liquid medium in a 300-mL conical flask, pick a little mycelium of Fusarium wilt of cucumber with an inoculation hook and put it in a sterilized culture bottle, set it on a shaker at 26℃~28℃, 200r / min, shaking culture for 6 days, the fermentation broth was centrifuged at 5000r / min for 10min, the mycelium was collected, vacuum freeze-dried at -53°C, 0.5mbar for 15h, the inactivated mycelium was aseptically used in a mortar Grind to powder, set aside;

[0021] Trichoderma harzianum strain L liquid fermentation: Trichoderma harzianum strain L is an endophytic fungus isolated from citridia, the strain has been preserved in the General Microbiology Center of the China Microorganism Culture Collection Management Committee, the preservation date is August 14, 20...

Embodiment 2

[0025] Cultivation of Fusarium wilt of cucumber: with embodiment 1;

[0026] Trichoderma harzianum strain L liquid fermentation: Trichoderma harzianum strain L is an endophytic fungus isolated from citridia, and the fermentation medium formula: its components and content (the mass contained in each liter of medium) are Cucumber Fusarium wilt bacteria Silk powder 90mg, peptone 30g, corn flour 10g, glucose 15g, bran 5g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; in the control, no inactivated mycelium powder of Fusarium wilt of cucumber was added, and the other components and contents were the same as the fermentation medium formula; 60mL of fermentation medium was packed in a 300mL triangular bottle, and 1mL of Trichoderma harzianum strain L spores were sucked with a sterile pipette Suspension (1×10 6 1 / mL) in a sterilized culture bottle, placed on a shaker at 28 °C, 180 r / min, and shaken for 96 h, the fermentation broth was centrifuged at 5000 r / min for 10 min, and the...

Embodiment 3

[0030]Cultivation of Fusarium wilt of cucumber: with embodiment 1;

[0031] Trichoderma harzianum strain L liquid fermentation: Trichoderma harzianum strain L is an endophytic fungus isolated from citridia, and the fermentation medium formula: its components and content (the mass contained in each liter of medium) are Cucumber Fusarium wilt bacteria Silk powder 90mg (added after 24 hours of liquid fermentation of Trichoderma harzianum), peptone 30g, corn flour 10g, glucose 15g, bran 5g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; in the control, no inactivated mycelium powder of Fusarium wilt of cucumber was added, and the other components and contents were the same as the fermentation medium formula; 60mL of fermentation medium was packed in a 300mL triangular bottle, and 1mL of Trichoderma harzianum strain L spores were sucked with a sterile pipette Suspension (1×10 6 1 / mL) in a sterilized culture bottle, placed on a shaker at 28 °C, 180 r / min, and shaken for 108 h, t...

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Abstract

The invention discloses a culture medium for producing trichodermin by trichoderma harziarum strain L through fermentation, and belongs to the technical field of microorganisms. The method comprises the following steps of: adding inactivated Fusarium oxysporiumf.sp.cucumeris mycelium powder into a culture medium for the trichoderma harziarum strain L for fermentation culture; centrifuging fermentation liquor; and analyzing the content of the trichodermin in the fermentation liquor by adopting a gas chromatogram, wherein the concentration of the trichodermin in the fermentation liquor after the inactivated Fusarium oxysporiumf.sp.cucumeris mycelium powder is added is improved by several times than that of a contrast. The invention provides a feasible method for improving the fermentation level of producing the trichodermin of the trichoderma harziarum strain L.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for increasing the level of trichoderma produced by Trichoderma harzianum. Background technique [0002] The titer in microbial fermentation is often the bottleneck that determines the production cost and realizes industrialized production. Genetic modification of strains, optimization of medium and culture conditions, etc. are common methods to improve the level of hormones. In recent years, fungal elicitors have been widely used in the study of plant cell culture and microbial fermentation to produce secondary products. Fungal elicitors are mainly fungal cell surface structures or secretions, which can be fungal mycelium, mycelium degradation products, fermentation broth, fungal secretions, etc., mainly including: polysaccharides, chitosan, β- 1-3-glucan, acetyl glucuronic acid, glycoprotein, protein, short peptide, unsaturated fatty acid, etc. There are few ...

Claims

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Application Information

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IPC IPC(8): A01P3/00C12P7/62A01N63/04C12R1/885C12R1/77
Inventor 申屠旭萍俞晓平董胜张郝培应边亚琳
Owner CHINA JILIANG UNIV
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