Culture medium for culturing mesenchymal stem cells

Abstract

The invention discloses a culture medium for culturing mesenchymal stem cells. The culture medium comprises improved minimum medium IMDM, cell growth factor combination, insulin, trace elements, lipid substances, vitamin substances, hormone substances, protein molecules, amino acid substances and other compounds. The culture medium provided by the invention does not contain serum or plasma (comprising animal or human serum or plasma), thus, when the system is used for culturing the mesenchymal stem cells, zoonosis caused by the animal serum or plasma in the cell therapy process can be avoided, and the risk of human immunity reaction which is possibly caused in the cell therapy process can be avoided. The culture medium has specific chemical constituent, thus the possibility of different cultured mesenchymal stem cell properties caused by the difference of culture systems can be avoided. The culture medium is not only suitable for culturing the mesenchymal stem cells of human marrow and umbilical cord resources but also suitable for culturing the mesenchymal stem cells of other tissues or other animals, and has the advantage of wide application range.

Description

technical field [0001] The invention belongs to the culture medium formula in the field of cell and tissue culture, and relates to a serum-free culture medium formula with clear chemical components. The culture medium is especially suitable for culturing human mesenchymal stem cells in vitro. Background technique [0002] Mesenchymal stem cells, whose English name is mesenchymal stem cells (MSC), also have other names, such as bone marrow stromal cells, adipose stem cells, etc. -Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 2006;8(4):315-7 ). In the bone marrow, MSCs belong to non-hematopoietic stem cells, have the ability to differentiate into osteoblasts, chondrocytes, and adipocytes, and have immune regulation and hematopoietic support functions. Recent studies have shown that MSCs are also pr...

AI Technical Summary

Problems solved by technology

Therefore, it can be considered that there may be many uncertain factors in the stability of MSC culture and the maintenance of stem cell pluripotency with serum substitutes such as bFGF-ITSE and hydrocortisone.

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