Application of asarum extract in preventing and controlling agricultural mites
A technology of two-spotted spider mites and extracts, applied in the application, acaricide, animal repellent and other directions, can solve the problems such as the use of Asarum extract that has not been found yet, achieve difficult drug resistance, expand the scope of application, and simple extraction easy effect
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experiment example 1
[0011] Experimental Example 1. Determination of Acaricidal Activity of Asarum Extract
[0012] The contact biological activity of Tetranychus urticae was determined by slide immersion method, a standard method recommended by FAO.
[0013] Experimental Materials:
[0014] Obtain the Herba Asarum extract of 95% ethanol as follows: 500 grams of dried Asarum herb are pulverized, passed through a 40 mesh sieve, extracted at room temperature with 5 times the amount of 95% ethanol for 4 days, and filtered to obtain the extract. Add 5 times the amount of 95% ethanol to the filter residue for leaching, and filter. After doing this three times, the three extracts were combined and concentrated under reduced pressure to obtain the Asarum extract of the present invention with a yield of 3.65%. Then weigh 0.2g of the above Asarum extract, add 1% of the total volume of the extract in acetone to dissolve, stir with 0.5% Tween-80, mix well, dilute with distilled water, and make the concentr...
experiment example 2
[0021] Experimental example 2. Determination of ovicidal activity of Asarum extract
[0022] The preparation of the spider mites used in the experiment was the same as that in Experimental Example 1, and the experimental solution of asarum extract for killing adults in Experimental Example 1 was diluted 10 times for this experiment.
[0023] experimental method
[0024] Refer to the standard method of ovicide recommended by FAO—leaf residual poison method to measure the ovicidal biological activity of Tetranychus urticae with slight modifications. Collect fresh cowpea leaves, cut off the petiole by 0.2 cm, and wrap wet cotton balls on the petiole, put it in a petri dish with a layer of wet cotton, cover the both sides of the blade with wet cotton to make the blade close to the cotton, and use zero Pick up 20 female adult mites to each leaf with a No. 2 soft brush, place them at 25±1°C and allow them to lay eggs, remove the female adults after 24 hours, check and record the nu...
Embodiment 1
[0033] The preparation of embodiment 1 Asarum extract
[0034] Grind 500 grams of dried Asarum herb, pass through a 40-mesh sieve, extract with 5 times the amount of 95% ethanol at room temperature for 5 days, and filter to obtain the extract. Add 5 times the amount of 95% ethanol to the filter residue for leaching, and filter. After doing this three times, the three extracts were combined and concentrated under reduced pressure to obtain the Asarum extract of the present invention with a yield of 3.65%.
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