Xanthomonas sp.SN-58 and method for preparing xanthan gum by using xanthomonas sp.SN-58
A technology of Xanthomonas and xanthan gum, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, mutant preparation, etc. In order to solve the problems of product instability and application field limitations, high product quality and improve product quality
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Embodiment 1
[0027] Mutation and Screening of Xanthomonas sp. SN-58
[0028] ⑴ Bacterial culture Take the original strain of Xanthomonas and inoculate it in a triangular flask filled with seed medium, and culture it with shaking at 32±1°C for 26 hours. The formula of the seed shaker flask: 0.8-1.0% sucrose, 0.5-0.6% soybean peptone , beef extract 0.1-0.3%, sodium chloride 0.1-0.2%, and the balance is water;
[0029] (2) Preparation of bacterial suspension Take the above seed liquid in a centrifuge tube, centrifuge at 3000r / min for 10min, discard the supernatant, add sterile water to shake and wash, centrifuge for 10min, discard the supernatant, then add sterile water, shake uniform;
[0030] (3) Mutagenesis Put the bacterial suspension in a sterile petri dish, put a magnetic stirring bar inside, place the electromagnetic stirrer under the ultra-clean workbench ultraviolet lamp, and irradiate for 0.5-1min at a distance of 28-32cm;
[0031] ⑷Cultivation in the dark box Take the mutagenized...
Embodiment 2
[0035] Fermented seed tank formula (by weight): 1.0% glucose, 0.5% soybean peptone, 0.3% beef extract, 0.2% sodium chloride, and the balance is water;
[0036] Fermentation tank formula (by weight): 4.0% sucrose, 0.5% soybean peptone, 0.2% citric acid, and the balance is water.
[0037] (1) Fermentation steps:
[0038] Inoculate Xanthomonas strains into seed tanks for primary and secondary seed cultivation, and cultivate them for 16 hours at a temperature of 32°C and a pH value of 7.4, and then transfer them to a fermenter at a ratio of 1:10 for cultivation. The temperature is 32°C, the pH value is 7.3-7.7, and the incubation time is 65 hours to obtain the fermentation broth; the pH of the fermentation process is regulated by feeding NaOH, and the amount of addition is based on the standard of maintaining the pH of the fermentation broth at 7.5±0.2;
[0039] (2) Fermentation broth extraction steps:
[0040] Add 0.5% potassium chloride to the above-mentioned fermentation broth,...
Embodiment 3
[0042] Fermented seed tank formula (by weight): 0.8% glucose, 0.6% soybean peptone, 0.2% beef extract, 0.1% sodium chloride, and the balance is water;
[0043] Fermentation tank formula (by weight): 3.5% sucrose, 0.6% soybean peptone, 0.1% citric acid, and the balance is water.
[0044] (1) Fermentation steps:
[0045] Inoculate Xanthomonas strains into seed tanks for primary and secondary seed cultivation, and cultivate them for 18 hours at a temperature of 34°C and a pH value of 7.6, and then transfer them to a fermenter at a ratio of 1:10 for cultivation. The temperature is 34°C, the pH value is 7.3-7.7, and the incubation time is 70 hours to obtain the fermentation broth; the pH of the fermentation process is regulated by feeding KOH, and the amount of addition is based on the standard of maintaining the pH of the fermentation broth at 7.5±0.2;
[0046] (2) Fermentation broth extraction steps:
[0047] Add 0.7% potassium chloride to the above fermented liquid, stir for 2...
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