Anti-saccharification agent

A technology of saccharifying agent and cosmetic composition, applied in the directions of cosmetics, cosmetic preparations, preparations for skin care, etc., can solve the problems of difficult decomposition reaction, accumulation of saccharification products, impaired protein function, etc., so as to improve skin aging or aging , The effect of inhibiting glycation reaction and inhibiting damage

Inactive Publication Date: 2011-05-18
ROHTO PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The glycosylation reaction of protein also occurs in the living body, but when the high blood sugar state continues due to diabetes, etc., or the decomposition reaction i

Method used

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Embodiment

[0183] Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.

reference example 1

[0184] Reference Example 1 (Promotion of AGEs generation by ultraviolet rays)

[0185] A collagen-gel was prepared using a collagen-gel culture kit (trade name: Cellmatrix, manufactured by Nitta Gelatin Co., Ltd.). Next, the collagen gel was incubated at 37° C. for two weeks in a ribose solution having a final concentration of 50 mM dissolved in PBS to perform a saccharification reaction. During this incubation, the irradiation intensity was 5J / cm 2 Conditional exposure to UVA.

[0186] Then, the collagen-gel was dissolved by collagenase treatment, and the amount of AGEs produced was measured by ELISA using the solution. For the measurement, an AGEs antibody (anti-AGEs monoclonal antibody, clone number 6D12, peroxidase-labeled, Transgenic) was used, and TMB reagent (manufactured by Dako) was used for color development.

[0187] In addition, in the above-mentioned method, except not having performed UVA irradiation, the same operation was performed, and this was used as a ...

reference example 2

[0194] Reference Example 2 (Promotion of CMA production by ultraviolet rays)

[0195] A collagen-gel was prepared using a collagen-gel culture kit (trade name: Cellmatrix, manufactured by Nitta Gelatin Co., Ltd.). Next, the collagen gel was incubated at 37° C. for two weeks in a ribose solution having a final concentration of 50 mM dissolved in PBS to perform a saccharification reaction. During this incubation, the irradiation intensity was 5J / cm 2 Conditional exposure to UVA.

[0196] Then, the collagen-gel was dissolved by collagenase treatment, and the amount of carboxymethylarginine (CMA), which is a kind of AGEs, was measured by ELISA using the solution. AGEs antibody (monoclonal anti-CMA antibody (3F5), donated by Lecturer Nagai, Japan Women's University) was used as the primary antibody, anti-mouse immunoglobulin / HRP (manufactured by Dako) was used as the secondary antibody, and TMB reagent (manufactured by Dako) was used as the secondary antibody ) to make it colo...

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Abstract

The invention provides the application of extracts of buddleja blades, and the extracts of buddleja blades are used to produce anti-saccharification agents, anti-aging agents, skin collagen damage inhibitors, or agents for inhibiting, preventing or improving the decrease in skin firmness or elasticity or skin wrinkles.

Description

technical field [0001] The present invention relates to an anti-glycation agent, anti-aging agent, skin collagen damage inhibitor, skin firmness or elasticity decrease, or skin wrinkle suppression, prevention or improvement agent used as a cosmetic composition or a food composition, and an agent for use in applications for the manufacture of these substances. Background technique [0002] A glycation reaction (Maillard reaction) occurs between reducing sugars such as glucose and protein to produce glycation products. The glycosylation reaction of protein also occurs in the living body, but when the high blood sugar state continues due to diabetes, etc., or the decomposition reaction is difficult due to aging, the reaction is inclined to the production of glycosylated products, resulting in impaired protein function, or glycosylated products accumulate. The glycation products eventually become advanced glycation end products (Advanced glycation end products; hereinafter som...

Claims

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Application Information

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IPC IPC(8): A61K8/97A23L1/29A61Q19/08A61Q19/00A61Q17/04A61K127/00A23L33/00
Inventor 林裕也须田一真永井龙儿
Owner ROHTO PHARM CO LTD
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