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Application method for medicament based on NAD<+> and NADH

An application method and drug technology, applied in the field based on NAD+ (nicotinamide adenine dinucleotide), can solve problems such as no direct application in the medical industry

Inactive Publication Date: 2011-05-18
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to basic research, NAD + Currently there is no direct application in the medical industry

Method used

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  • Application method for medicament based on NAD&lt;+&gt; and NADH
  • Application method for medicament based on NAD&lt;+&gt; and NADH
  • Application method for medicament based on NAD&lt;+&gt; and NADH

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take NAD + Drugs with NADH as the main component reduce the survival of neuronal tumor (Neuro2A) cells

[0021] Utilize Dulbecco's Modified Eagle Medium containing 10% (volume fraction) calf serum (see Dos-Anjos et al. in "Analytical Biochemistry" ("Analytical Chemistry") 378 series 99-101 pages entitled "Quantitative gene expression Analysis in a brain slice model: influence of temperature and incubation media" (a digital gene analysis of a brain slice model: the influence of temperature and culture medium) article) will NAD + (Sigma Company) and NADH formulation containing 10mM NAD + and a solution of 1 μM NADH, and then dilute the prepared 10 mM NAD with Dulbecco's Modified Eagle Medium containing 10% (volume fraction) calf serum + , and then get 0.1mM NAD + and 0.01 μM NADH, and 1 mM NAD + and 0.1 μM NADH as the main component of the drug, and then these concentrations of NAD + and NADH were added to neuron tumor (Neuro2A) cells (Cell Resource Center, Shanghai ...

Embodiment 2

[0023] Take NAD + Drugs with NADH as major components reduce survival of glioma (C6) cells

[0024] Utilize Dulbecco's Modified Eagle Medium containing 10% (volume fraction) calf serum (see Dos-Anjos et al. in "Analytical Biochemistry" ("Analytical Chemistry") 378 series 99-101 pages entitled "Quantitative gene expression Analysis in a brain slice model: influence of temperature and incubation media" (a digital gene analysis of a brain slice model: the influence of temperature and culture medium) article) will NAD + (Sigma Company) and NADH formulation containing 10mM NAD + and a solution of 1 μM NADH, and then dilute the prepared 10 mM NAD with Dulbecco's Modified Eagle Medium containing 10% (volume fraction) calf serum + , and then get 0.1mM NAD + and 0.01 μM NADH, and 1 mM NAD + and 0.1 μM NADH as the main component of the drug, and then these concentrations of NAD + and NADH were added to glioma cells (Cell Resource Center, Shanghai Academy of Biological Sciences, Chi...

Embodiment 3

[0026] Take NAD+ Drugs with NADH as the main component reduce survival of breast cancer (MCF-7) cells

[0027] Utilize Dulbecco's Modified Eagle Medium containing 10% (volume fraction) calf serum (see Dos-Anjos et al. in "Analytical Biochemistry" ("Analytical Chemistry") 378 series 99-101 pages entitled "Quantitative gene expression Analysis in a brain slice model: influence of temperature and incubation media" (a digital gene analysis of a brain slice model: the influence of temperature and culture medium) article) will NAD + (Sigma Company) and NADH formulation containing 10mM NAD + and a solution of 1 μM NADH, and then dilute the prepared 10 mM NAD with Dulbecco's Modified Eagle Medium containing 10% (volume fraction) calf serum + , and then get 0.1mM NAD + and 0.01 μM NADH, and 1 mM NAD + and 0.1 μM NADH as the main component of the drug, and then these concentrations of NAD + and NADH were added to breast cancer (MCF-7) cells (Cell Resource Center, Shanghai Institute ...

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Abstract

The invention provides an application method for a medicament based on NAD<+> and NADH, belonging to the technical field of biology. The method comprises the following steps: mixing powdery NAD<+> and NADH at a weight ratio of 0.01-99.99%, thus forming a medicament containing 0.001mM NAD<+> and 0.001-10mM NADH; and adding the medicament obtained in the step 1 into malignant tumor cells subjected to isolated culture, and culturing. The medicament provided by the invention can be used for killing glia tumor cells, neuron tumor cells, gastric cancer tumor cells, kidney cancer tumor cells or breast cancer tumor cells.

Description

technical field [0001] The present invention relates to a medicine preparation and application method in the field of biotechnology, specifically a NAD-based + (nicotinamide adenine dinucleotide; nicotinamide adenine dinucleotide, referred to as NAD + ) and NADH (nicotinamide adenine dinucleotide (reduced state); nicotinamide adenine dinucleotide, reduced form, NADH for short). Background technique [0002] Malignant tumors are one of the most serious frequently-occurring diseases that seriously endanger the health of the people. Currently used methods such as radiotherapy and chemotherapy have adverse side effects such as large damage to normal tissues and cells. Therefore, it is of great clinical significance to find drugs that do not kill normal tissues but have killing or growth-inhibiting effects on tumor cells. [0003] NAD + Both NADH and NADH are coenzymes that transfer hydrogen ions in the action of dehydrogenase. It appears in many metabolic reactions of cells,...

Claims

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Application Information

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IPC IPC(8): C12N5/09A61K31/7084A61P35/00
Inventor 殷卫海夏伟梁马英鑫赵翠平洪韵仪韩瑾陈贺愈
Owner SHANGHAI JIAO TONG UNIV
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