Method for tissue artificial culture of hymeniacidon perleve by using program for temperature regulation and control

Abstract

The invention relates to a method for tissue artificial culture of hymeniacidon perleve by using a program for temperature regulation and control. By using the method, a temperature control system is established artificially, the influence on the sponge growth due to seasonal temperature changes is avoided, the seasonal growth mode of the sponge in a natural sea area is changed into a continuous growth mode under an artificially controllable condition, and biomass changes, tissue form changes and internal structure changes in the growth cycle of the sponge are inspected. The invention provides a set of complete temperature regulation and control method for reactor culture of a sponge explant, makes the over-expression of active substances in a spongy body through further metabolic regulation and control under a controllable condition possible, and provides a novel and comprehensive object system for the reactor culture of the sponge explant and researches on a cell production biogenic siliceous material.

Description

technical field [0001] The invention relates to the collection, breeding and growth cycle research of the luxuriant membrane sponge. Specifically, it is a method of artificially cultivating hymeniacidon perlevis tissue by using programmed temperature regulation. By artificially constructing a temperature control system, it breaks the influence of seasonal temperature changes on the growth of hymeniacidon perlevis, and puts the sponge in the natural sea area. The seasonal growth pattern becomes a continuous growth pattern under artificially controllable conditions. The invention provides a complete set of programmed temperature control method for sponge explant reactor culture, can artificially control the growth cycle of the sponge under laboratory conditions, and the sponge tissue in different periods can be used for different research purposes. Background technique [0002] Sponges are the lowest multicellular animals. They contain a variety of active compounds with novel...

AI Technical Summary

Problems solved by technology

However, in the development of sponge drugs, a problem that cannot be ignored is that the content of active substances in the sponge is very low

[0004] So far, the cultivation of sponge explant reactors to obtain a large amount of sponge biomass has not been successful, and the method of using programmed temperature to regulate the growth cycle of luxuriant membrane sponges has not been reported.

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