Application of active burdock oligosaccharide to aquaculture

A burdock oligosaccharide, aquaculture technology, applied in the directions of application, organic active ingredients, medical preparations containing active ingredients, etc., can solve problems such as unreported, reduce pollution, improve non-specific defense mechanisms, and simple production technology Effect

Inactive Publication Date: 2011-06-15
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a kind of active oligosaccharides, the research of burdock oligosaccharides in animal feed additives has just started, and the application in aquaculture has not been reported at home and abroad.

Method used

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  • Application of active burdock oligosaccharide to aquaculture
  • Application of active burdock oligosaccharide to aquaculture
  • Application of active burdock oligosaccharide to aquaculture

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The juvenile turbot used in the experiment was provided by Yantai Development Zone Tianyuan Aquatic Products Co., Ltd. 1200 healthy and homogeneous domesticated turbot were randomly divided into 5 groups, each with a parallel group, using different baits, and carried out according to the normal feeding and management procedures. Group 5 was the control group, fed with basic feed. The 1st, 2nd, 3rd and 4th groups were test groups, fed with feed containing 0.5%, 1.0%, 2.0% and 5.0% burdock oligosaccharides respectively, and carried out running water aerated culture in net cages (120cm×100cm×100cm). The aquaculture water is mixed with underground seawater and natural seawater, and then flows into the test cages through a disposable sand filter. Water quality conditions during the test: dissolved oxygen DO is 6.0-8.0mg / L, pH is 7.8-8.1, temperature T is 18-22°C, and salinity S is 30-31. The test was carried out in the breeding workshop of Tianyuan Aquatic Products Co., Lt...

Embodiment 2

[0044] After the growth test, the immunoassay was performed. Randomly select 10 fish from each group of test fish, dry their tails with gauze, collect blood from the tail vein with 1mL sterile syringes, mix them together, and divide them into 2 parts, one part is added pre-cooled Alsever to the syringe Anticoagulant, after taking blood, mix it in a 1.5mL Eppendorf tube to prepare anticoagulant blood, then add lymphocyte separation medium, separate hemorrhage lymph, and use it for the determination of leukocyte phagocytosis; the other part is stored in a refrigerator at 4°C Let stand for 12 hours, then centrifuge at 3000r / min for 10 minutes, and separate out the supernatant serum for later use.

[0045] Determination of non-specific immune factors: determination of acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LSZ), superoxide dismutase (SOD) and phenoloxidase (PO) activities in serum and hemolymph Phagocytic activity of blood cells in blood against Staphylococ...

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Abstract

The invention discloses application of active burdock oligosaccharide to aquaculture, particularly the application of the active burdock oligosaccharide serving as an immunopotentiator to aquaculture. The burdock oligosaccharide is natural oligosaccharide which is extracted and separated from burdock root and has better water solubility, better stability and higher purity, wherein the oligosaccharide content is more than 95 percent; the average molecular weight is 2,134Da; 12 furoic fructoses are connected through beta (2-1) glucosidic bonds; the tail end of the burdock oligosaccharide is of a linear straight chain structure that a glucopyranose is connected onto the fructose through an alpha (1-2) glucosidic bond; and degree of polymerization is 13. When the burdock oligosaccharide is used as a growth promoting agent and the immunopotentiator in agriculture, the addition amount of the burdock oligosaccharide accounts for 0.5 to 5.0 percent of the total weight of feeds. The active burdock oligosaccharide has the beneficial effects of promoting the growth of cultured animals, improving the nutrient constituents of animal bodies and improving the nonspecific immunity and the premunition of the animals, also has high activity in the aspect of resisting bacterial infection, has a simple, convenient and feasible using method and can be suitable for cultivation of various aquatic products.

Description

technical field [0001] The invention relates to the application of an active burdock oligosaccharide in aquaculture animals, in particular to the application of an active burdock oligosaccharide as a feed nutrient and an immune enhancer in aquaculture. Background technique [0002] With the increasing development of fishery production, my country's aquaculture has made great achievements, and the aquaculture output has continued to grow steadily, becoming the world's largest aquaculture country. However, in recent years, with the continuous strengthening of the degree of intensification, the increase of breeding density and the continuous deterioration of the breeding environment, the accompanying diseases have become more and more serious, which has caused huge economic losses to the breeding industry. Therefore, the healthy breeding and disease prevention of aquatic animals have attracted more and more attention. [0003] At present, there are mainly three methods for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P37/04A23K1/18A23K1/16A23K20/163A23K50/80
CPCY02A40/818
Inventor 郝林华孙丕喜李继业
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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