Cynoglossus semilaevis triploid fry mass inducing method

Abstract

The invention relates to a cynoglossus semilaevis triploid fry mass inducing method which comprises an inducing method and an authenticating method of a triploid fry. The inducing method comprises the steps of collection and insemination of an unfertilized egg, confirmation of triploid induction starting time and the confirmation of hydrostatic shock pressure and processing time; and the authenticating method comprises the steps of authentication with a ploidy tester and the analyzing authentication of chromosome. The method for producing cynoglossus semilaevis triploid fries massively by adopting hydrostatic induction is established at first; the proper start time and the hydrostatic shock condition of triploid induction are screened; and the triploid fry mass inducing method is established. The inducing rate of the triploid fry obtained by the method disclosed by the invention is as high as more than 95 percent. The method established by the invention has the characteristics of advancement, high efficiency, accuracy and reliability, has important application value in cynoglossus semilaevis triploid induction and sterile seedling production and has broad popularization and application prospect in fish cultivation and breeding.

Description

Technical field: [0001] The invention belongs to aquatic genetic breeding technology, and relates to a method for inducing triploid semi-smooth tongue sole by using a physical method. Background technique: [0002] Semi-smooth tongue sole (Cynoglossus semiliaevis) belongs to Pleuronectiformes, Cynoglossidae, and Cynoglossus. Warm water bottom fish. It is delicious, tender and nutritious, and is welcomed by consumers, and its market value is extremely high. Half-smooth tongue sole farming has been widely carried out in the coastal areas of my country, and its annual output value of the farming industry reaches 1.5-2 billion yuan. However, due to the large size difference between male and female semi-smooth tongue sole, the growth rate of males is slow, and the appearance and quality of large-scale commercial fish are affected by the bulging of the abdominal gonads of females after sexual maturity, which affects their commercial value, thus seriously affecting fish farming. ...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to establish a technical method for mass production of triploid fry of tongue sole by hydrostatic pressure induction, to achieve the purpose of industrialized production of triploid fry; to overcome the large size difference between male and female of tongue sole and the slow growth of males through the production of triploid fry , excessive development of female gonads, affecting the quality and appearance of commercial fish, so as to improve the production and economic benefits of semi-smooth tongue sole farming

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