Compound lightyellow sophora root preparation and application thereof
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A technology of Sophora flavescens and preparations, which is applied in the field of research and development of its pharmaceutical products, and can solve the problems of stagnant development and popularization of botanical fungicides and the urgent need for botanical fungicides
Inactive Publication Date: 2011-07-20
SHANDONG UNIV AT WEIHAI
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However, it is a pity that in recent years, the work on screening plants with antibacterial activity of fish-causing bacteria has deviated from its advantages. The blind pursuit of strong bactericidal activity has caused the screened plants to
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Embodiment 1
[0020] 1, a compound Sophora flavescens preparation is characterized in that being the preparation made by following weight proportioning:
[0022] 2. The preparation method of a compound Sophora flavescens preparation according to claim 1, characterized in that it comprises the following steps:
[0023] Step (1): take 30 g each of Sophora flavescens, Neem leaf and Willow leaf respectively, add 600 mL of ethanol respectively, shake and extract with a shaker for 24h, and centrifuge with a centrifuge to obtain supernatant and residue;
[0024] Step (2): adding 400 mL of ethanol to the residue in (1), leaching with a shaker for 18h, and centrifuging to obtain the supernatant and residue;
[0025] Step (3): add 200 mL of ethanol to the residues in (2) respectively, extract by shaking for 12h, centrifuge to obtain supernatant and residue, discard the residue, combine ...
Embodiment 2
[0030] 1, a compound Sophora flavescens preparation is characterized in that being the preparation made by following weight proportioning:
[0032] 2. The preparation method of a compound Sophora flavescens preparation according to claim 1, characterized in that it comprises the following steps:
[0033] Step (1): take 40g of Sophora flavescens, Neem leaf and Willow leaf respectively, add 800mL of ethanol respectively, shake and extract with a shaker for 24h, and centrifuge with a centrifuge to obtain supernatant and residue;
[0034] Step (2): add 600 mL of ethanol to the residue in (1), shake and extract with a shaker for 18h, and centrifuge to obtain supernatant and residue;
[0035] Step (3): add 400 mL of ethanol to the residues in (2) respectively, extract by shaking for 12h, centrifuge to obtain supernatant and residue, discard the residue, combine the sup...
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Abstract
The invention belongs to the technical field of research and development of medicinal products and relates to a compound lightyellow sophora root preparation and application thereof. The compound lightyellow sophora root preparation is prepared by the following steps of: adding ethanol into lightyellow sophora root, melia azedarach linn leaf and salix leaf respectively; oscillating and lixiviating by using a table concentrator; centrifuging by using a centrifugal machine to obtain supernate; extracting repeatedly for twice; combining the supernate of the three times; filtering; concentrating the filtrate under reduced pressure; drying to obtain extracts of the lightyellow sophora root, the melia azedarach linn leaf and the salix leaf; adding pounded garlic into a round-bottomed flask to perform steam distillation to obtain garlic essential oil; and preparing the compound lightyellow sophora root preparation from the lightyellow sophora root extract, the melia azedarach linn leaf extract, the salix leaf extract and the garlic essential oil. The compound lightyellow sophora root preparation has bacteriostasis on vibrio anguillarum, vibro harveyi, vibrio alginnolyficus and halophilicvibrio. The corresponding preparation can be developed to be used for preventing and treating vibriosis in the aquaculture process. The compound lightyellow sophora root preparation is suitable for industrialized production.
Description
technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products. The present invention relates to a compound Sophora flavescens preparation and its application. The product of the present invention has antibacterial effect on D31 (V. anuguillarum), D32 (V. harveyi), D61 (V. alginolyticus), D230 (Vibrio vulnificus) Therefore, corresponding formulations can be developed for preventing and treating vibrosis in the process of aquaculture, and the product of the invention is suitable for industrial production. Background technique [0002] In recent years, my country has vigorously developed the marine aquaculture industry, but due to the rapid expansion of aquaculture area, high aquaculture density and improper management, the breeding of fish diseases has caused huge economic losses. Vibrio disease caused by D31 (V. anuguillarum), D32 (V. harveyi), D61 (V. alginolyticus), D230 (Vibrio vulnificus) is recognized Vibrio...
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