Process for extracting and purifying flavonoids in grosvener siraitia plants
An extraction process, the technology of Luo Han Guo, applied in the direction of plant/algae/fungus/moss components, medical preparations containing active ingredients, plant raw materials, etc., to achieve the effect of low price, low cost and recyclable price
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Embodiment 1
[0021] Take 12 kg of Luo Han Guo plants, chop them up, add 40% ethanol to reflux extraction, and extract 3 times, the first time is 2 hours, the second and third times are 1 hour each, and the amount of ethanol is 10 times the mass of the medicinal material. amount, 8 times the amount, 6 times the amount, and combine the extracts. Then the extract was concentrated to twice the mass of the medicinal material, and the sample medicinal solution was obtained by suction filtration. Use 1 mol / L HCl to adjust the pH of the drug solution to 4, and the concentration of the sample solution is 0.4 g / mL, and the sample flow rate is 3 Bv / h for adsorption (resin bed diameter-to-height ratio is 1:8). After the adsorption is completed, Use 3 times the resin volume of distilled water to elute at a flow rate of 4 Bv / h, then use 5 times the resin column volume to elute with 30% ethanol at a flow rate of 2 Bv / h, collect the eluate, and wash with ammonia water Adjust the pH of the liquid to 5-6. ...
Embodiment 2
[0023] Take 5 kg of Luo Han Guo plants and chop them up, add 8 times the mass of medicinal materials with 60% ethanol for reflux extraction, extract once, and the extraction time is 2 hours. The extract is then concentrated to three times the mass of the medicinal material, and the filtrate is obtained after standing and filtering. Use 1 mol / L HCl to adjust the pH of the filtrate to 3-5, and pass the sample solution at a concentration of 0.1 g / mL at a flow rate of 4 Bv / h through a macroporous adsorption resin column. h flow rate for elution, and then use 4 times the column bed volume of 50% ethanol to elute at a flow rate of 1 Bv / h, adjust the pH of the liquid to 5-6 with ammonia water, and obtain the ethanol eluent. Concentrate the eluent and recover ethanol, and dry the concentrated solution to obtain the crude product of the flavonoid component extract of the Luo Han Guo plant. Then the extract powder is dissolved 5 times in ethanol with a concentration of 85%, and the amo...
Embodiment 3
[0025] Take 10 kg of Luo Han Guo plants, chop them up, add 80% ethanol to reflux extraction, and extract twice, the first time is 2 hours, the second time is 1 hour, and the amount of ethanol is 10 times and 6 times the mass of the medicinal material respectively. , combined extracts. The extract is then concentrated to 5 times the mass of the medicinal material, and the filtrate is obtained after standing and filtering. Adjust the pH of the filtrate to 4 with 1mol / L HCl, and pass the sample solution at a concentration of 0.5g / mL at a flow rate of 5 Bv / h through a macroporous adsorption resin column, and then use distilled water twice the volume of the resin at a flow rate of 4 Bv / h Elute, and finally use 40% ethanol with a concentration of 8 times the column bed volume for elution at a flow rate of 3Bv / h, and adjust the pH of the drug solution to 5-6 with ammonia water to obtain an eluent. Concentrate the eluent and recover ethanol, and dry the concentrated solution to obtai...
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