Process for extracting and purifying flavonoids in grosvener siraitia plants

An extraction process, the technology of Luo Han Guo, applied in the direction of plant/algae/fungus/moss components, medical preparations containing active ingredients, plant raw materials, etc., to achieve the effect of low price, low cost and recyclable price

Inactive Publication Date: 2011-08-03
湖北汇特生物医药技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention uses ethanol as the extraction solvent, and the extract is purified by macroporous adsorption resin, and the flavonoid content of the obtained extract is relatively high. There is no relevant data report at home and abroad for this extraction and purification technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 12 kg of Luo Han Guo plants, chop them up, add 40% ethanol to reflux extraction, and extract 3 times, the first time is 2 hours, the second and third times are 1 hour each, and the amount of ethanol is 10 times the mass of the medicinal material. amount, 8 times the amount, 6 times the amount, and combine the extracts. Then the extract was concentrated to twice the mass of the medicinal material, and the sample medicinal solution was obtained by suction filtration. Use 1 mol / L HCl to adjust the pH of the drug solution to 4, and the concentration of the sample solution is 0.4 g / mL, and the sample flow rate is 3 Bv / h for adsorption (resin bed diameter-to-height ratio is 1:8). After the adsorption is completed, Use 3 times the resin volume of distilled water to elute at a flow rate of 4 Bv / h, then use 5 times the resin column volume to elute with 30% ethanol at a flow rate of 2 Bv / h, collect the eluate, and wash with ammonia water Adjust the pH of the liquid to 5-6. ...

Embodiment 2

[0023] Take 5 kg of Luo Han Guo plants and chop them up, add 8 times the mass of medicinal materials with 60% ethanol for reflux extraction, extract once, and the extraction time is 2 hours. The extract is then concentrated to three times the mass of the medicinal material, and the filtrate is obtained after standing and filtering. Use 1 mol / L HCl to adjust the pH of the filtrate to 3-5, and pass the sample solution at a concentration of 0.1 g / mL at a flow rate of 4 Bv / h through a macroporous adsorption resin column. h flow rate for elution, and then use 4 times the column bed volume of 50% ethanol to elute at a flow rate of 1 Bv / h, adjust the pH of the liquid to 5-6 with ammonia water, and obtain the ethanol eluent. Concentrate the eluent and recover ethanol, and dry the concentrated solution to obtain the crude product of the flavonoid component extract of the Luo Han Guo plant. Then the extract powder is dissolved 5 times in ethanol with a concentration of 85%, and the amo...

Embodiment 3

[0025] Take 10 kg of Luo Han Guo plants, chop them up, add 80% ethanol to reflux extraction, and extract twice, the first time is 2 hours, the second time is 1 hour, and the amount of ethanol is 10 times and 6 times the mass of the medicinal material respectively. , combined extracts. The extract is then concentrated to 5 times the mass of the medicinal material, and the filtrate is obtained after standing and filtering. Adjust the pH of the filtrate to 4 with 1mol / L HCl, and pass the sample solution at a concentration of 0.5g / mL at a flow rate of 5 Bv / h through a macroporous adsorption resin column, and then use distilled water twice the volume of the resin at a flow rate of 4 Bv / h Elute, and finally use 40% ethanol with a concentration of 8 times the column bed volume for elution at a flow rate of 3Bv / h, and adjust the pH of the drug solution to 5-6 with ammonia water to obtain an eluent. Concentrate the eluent and recover ethanol, and dry the concentrated solution to obtai...

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PUM

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Abstract

The invention relates to the technical field of the extraction and purification of flavonoids in medicinal plants, in particular to a process for extracting and purifying flavonoids in grosvener siraitia plants. In the process, ethanol is used as extracting solvent, and extracting solution is purified by macroporous adsorbent resin. The process is characterized by comprising the following steps of: drying, chopping, performing reflux extraction by using the ethanol, filtering, concentrating, adsorbing by using a column, eluting by using water, eluting by using the ethanol, concentrating and drying, dissolving by using the ethanol, filtering, and concentrating and drying. The process has a simple flow, effective substances are high in content, the production cost can be reduced, energy can be saved, and environmental pollution can be controlled.

Description

technical field [0001] The invention relates to the technical field of extraction and purification of flavonoid components of medicinal plants, in particular to an extraction and purification process of flavonoid components of Luo Han Guo plants. Background technique [0002] Luo Han Guo is a plant of the genus Luo Han Guo in the family Cucurbitaceae ( Siraitia grosvenorii (Swingle) C. Jeffrey ex Lu et Z. Y. Zhang), a traditional Chinese medicine. This traditional Chinese medicine resource is also a unique economic plant in Guangxi. It has high nutritional value and is widely used in medicine, beverages and condiments. This plant has been used in folk medicine for more than 300 years in Guangxi. It was recorded in the 2010 edition of "Chinese Pharmacopoeia". Blood sugar, anti-oxidation and other biological activities, the Ministry of Health included it in the first batch of "list of varieties that are both food and medicine". [0003] Luo Han Guo has tall plants and dens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/42A61P3/10A61P39/06A61K125/00A61K127/00A61K135/00
Inventor 刘焱文吴和珍周莉卢觉晓左杰黄赤夫余波
Owner 湖北汇特生物医药技术有限公司
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