Corayceps militaris polysaccharide two-stage fermentation synthesis process

A technology for the synthesis of Cordyceps militaris and a synthetic process, which is applied in the field of synthesizing polysaccharides from Cordyceps militaris by a biological two-stage fermentation method, which can solve the problems of low yield of biologically active substances, and achieve the effects of increased yield, significant benefits, and simple operation

Inactive Publication Date: 2014-03-05
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it mainly produces bacteria, and the production of biologically active substances (polysaccharides) is relatively low

Method used

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  • Corayceps militaris polysaccharide two-stage fermentation synthesis process
  • Corayceps militaris polysaccharide two-stage fermentation synthesis process
  • Corayceps militaris polysaccharide two-stage fermentation synthesis process

Examples

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Effect test

Embodiment 1

[0038] The present invention is a two-stage fermentation synthesis process of Cordyceps militaris polysaccharide, which comprises the following steps:

[0039] a. Preservation of strains: The cordyceps strains grown on potato slant medium are stored in a refrigerator at 4°C for later use.

[0040] b. Activation of slant strains:

[0041] Strain activation: the Cordyceps militaris strain preserved in the above step a is transferred to the potato slant medium with a sterilized inoculating shovel, and the activated Cordyceps militaris strain is obtained by culturing in a 25° C. incubator for 7 days for use.

[0042] c. Seed culture: The Cordyceps militaris strain activated in the above step b is used to cut about 1cm2 of the inclined hyphae with culture medium with sterilized inoculation and transfer them into a 250mL Erlenmeyer flask containing 50mL seed culture medium, and place in a shaker Cultivate with shaking at 25°C and 150r / min for 4-5 days to obtain seed liquid for later use.

[...

Embodiment 2

[0058] The two-stage fermentation culture process method of this embodiment is different from the embodiment 1 in that the nutrient components and the added amount of the fermentation medium are as follows:

[0059]

Embodiment 3

[0061] The two-stage fermentation culture process method of this embodiment is different from that in Example 1 in that during the fermentation culture process, the seed liquid is inserted into the fermentation tank containing the fermentation medium at a 5% inoculum amount. The fermentation culture process includes two stages :The fermentation conditions of the first stage include: fermentation tank temperature 25℃, ventilation volume 1vvm, stirring speed 100-150rpm, fermentation tank pressure 0.05MPa, culture for 6-7 days; second stage fermentation conditions: fermentation tank temperature 25℃ ,Ventilation volume 0vvm (not aeration), culturing for 1-2 days under the condition of stirring speed 20-30rpm.

[0062] Compared with the prior art, the present invention has the following advantages: According to the growth and metabolism characteristics of Cordyceps militaris, the present invention designs and establishes a two-stage fermentation and culture process to synthesize Cordyc...

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Abstract

The invention belongs to the microorganism field, particularly relates to a corayceps militaris polysaccharide two-stage fermentation synthesis process; the process comprises the following steps: corayceps militaris strains are vaccinated on a potato slant culture medium, are cultured at the constant temperature and then are put in a conical flash containing a seed culture medium for shaking culture; then the corayceps militaris strains are put in a fermentation tank containing a fermentation medium, the fermentation process is as follows: the corayceps militaris is cultured at constant temperature for four to five days in ventilating and stirring condition, which is mainly used for providing a culture condition suitable for growing of corayceps militaris thallus so that the corayceps militaris grows rapidly; then the corayceps militaris is cultured for two to three days at the constant temperature in the condition of ventilation and slightly stirring, a culture solution is centrifuged, and ethanol in two times of volume of is added into an obtained liquid supernatant which is mixed and put up for the night at the temperature of 4 DEG C; and precipitates obtained through secondary centrifugation is dried so as to obtain the corayceps militaris. The process is reasonable in design and simple in operation, is suitable for large-scale industrial production and can solve the situation of drug source shortage and has obvious benefits.

Description

Technical field [0001] The invention relates to a process for artificially culturing and synthesizing cordyceps polysaccharides, in particular to a method for synthesizing cordyceps polysaccharides by adopting a biological two-stage fermentation method. Background technique [0002] Cordyceps militaris (commonly known as Cordyceps militaris) is a complex of insects and bacteria formed by the larvae of the Hepium genus of the Lepidoptera Hepteridae parasitized by Trichospora chinensis. It is a valuable medicinal material under national secondary protection. It is listed as one of China's "Three Treasures of Traditional Chinese Medicine". It contains a variety of active ingredients (such as cordyceps mycelium, polysaccharides, cordycepin, etc.), which has the effects of enhancing immunity, anti-tumor, anti-oxidation and lowering blood sugar, and has great market demand. [0003] However, the growth cycle of wild cordyceps is too long, and due to the excessive excavation of wild cord...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 崔建东张思孙立梅
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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