Preparation method for measuring biological active substance of feed protein of ruminants

A technology for biologically active substances and feed proteins, which is applied in the field of preparation of biologically active substances in the determination of ruminant feed proteins, can solve the problems of pollution, low accuracy, and ruminants are prone to diseases, and achieves simple and cheap operation, less pollution, and difficulty in effect of disease

Inactive Publication Date: 2011-08-17
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, since urea can be recirculated through saliva or direct diffusion back to the rumen to form nitrogen, it makes the calculation of the biological value of protein in ruminants more difficult to achieve
[0005] Since the 1970s, many countri

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Step 1: Separation of biologically active substances in rumen fluid: When feeding at 7 o'clock in the morning, select 3 adult cows, insert the oral rumen fluid extraction device into the rumen through the mouth, adjust the vacuum pressure of the vacuum pump at 15 Pa, and extract the rumen fluid at 60 Purpose filter, filter to remove feed residues, then transfer the rumen fluid containing biologically active substances to the container, and pass CO 2 An anaerobic environment is present during the guarantee period;

[0022] Step 2: Preparation of freeze-dried powder of biologically active substances in rumen fluid:

[0023] 1. Add a freeze-dried protective agent to the rumen fluid containing biologically active substances after anaerobic treatment in a ratio of 1:1, mix it to make a suspension, and then place the suspension at 0°C to equilibrate for 20 minutes;

[0024] 2. Place the balanced suspension at -30°C for 30 minutes, and perform the first pre-freezing to obtain frozen ...

Embodiment 2

[0028] Step 1: Separation of biologically active substances in rumen fluid: When feeding at 7 o'clock in the morning, select 4 adult cows, insert the oral rumen fluid extraction device into the rumen through the mouth, adjust the vacuum pressure of the vacuum pump at 30 Pa, and extract the rumen fluid for 60 Purpose filter, filter to remove feed residues, then transfer the rumen fluid containing biologically active substances to the container, and pass CO 2 An anaerobic environment is present during the guarantee period;

[0029] Step 2: Preparation of freeze-dried powder of biologically active substances in rumen fluid:

[0030] 1. Add the freeze-dried protective agent to the rumen fluid containing biologically active substances after anaerobic treatment in a ratio of 1:1, mix it to make a suspension, and then place the suspension at 2°C for 25 minutes to equilibrate;

[0031] 2. Place the balanced suspension at -33°C for 60 minutes, and perform the first pre-freezing to obtain froz...

Embodiment 3

[0035] Step 1: Separation of biologically active substances in rumen fluid: When feeding at 7 o'clock in the morning, select 5 adult cows, insert the oral rumen fluid extraction device into the rumen through the mouth, adjust the vacuum pressure of the vacuum pump at 50 Pa, and extract the rumen fluid at 60 Purpose filter, filter to remove feed residues, then transfer the rumen fluid containing biologically active substances to the container, and pass CO 2 An anaerobic environment is present during the guarantee period;

[0036] Step 2: Preparation of freeze-dried powder of biologically active substances in rumen fluid:

[0037] 1. Add a freeze-dried protective agent to the rumen fluid containing biologically active substances after anaerobic treatment at a ratio of 1:1, mix it to make a suspension, and then place the suspension at 4°C for 30 minutes to equilibrate;

[0038] 2. Place the balanced suspension at -35°C for 90 minutes, and perform the first pre-freezing to obtain frozen ...

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Abstract

The invention relates to a preparation method for measuring a biological active substance of feed protein of ruminants. The method comprises the following steps of: extracting rumen fluid of cattle or sheep, and filtering the rumen fluid to remove fed residues from the rumen fluid; and mixing liquid obtained by filtering and a freeze drying protective agent according to a ratio of 1:1, and putting the mixture into a vacuum freeze drying machine for freeze drying to prepare biological active substance powder. By the method, the problem that available protein in ruminant feed is measured only in the rumen fluid provided by rumen fistula animals or excrement of the ruminants is solved, so that the nutritional value of feed protein of the ruminants can be evaluated simply, conveniently, quickly and accurately, nutrient substances are provided for animals accurately, and diseases are prevented simultaneously.

Description

Technical field [0001] The invention relates to a preparation method for measuring biologically active substances, in particular to a preparation method for measuring biologically active substances of ruminant feed protein. Background technique [0002] As the main nutritional index of animal diet, protein is the main content of animal nutrition research. As the shortage of protein feed resources in the world is intensifying, and people’s requirements for the quality of livestock products and environmental protection continue to increase, it is of great significance to accurately assess the nutritional value of feed protein. [0003] For a long time, the evaluation of the nutritional value of ruminant protein often used the crude protein system or digestible protein system proposed by Mitchell (1951) to determine the biological value of protein. The crude protein system is evaluated based on the nitrogen content in the diet, which is limited to chemical analysis, and does not comb...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
Inventor 李元晓王玉琴王占彬达赖张勇法
Owner HENAN UNIV OF SCI & TECH
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