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Method for in-vitro induction of remineralization of dentin

A technology for remineralization and tooth body, applied in the field of biomimetic biomaterial engineering, can solve problems such as remineralization of very few gel systems, and achieve the effects of low cost, less time-consuming and simple method

Inactive Publication Date: 2013-01-09
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most gel systems focus on simulating enamel mineralization in vitro, and there are few reports on the use of gel systems to remineralize dentin

Method used

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  • Method for in-vitro induction of remineralization of dentin
  • Method for in-vitro induction of remineralization of dentin
  • Method for in-vitro induction of remineralization of dentin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1.1 Preparation of tooth slices

[0020] The collected third molars (with complete crowns and no caries and fillings) were cut into thin slices about 1 mm thick with a low-speed cutting machine perpendicular to the longitudinal axis of the teeth, and cooled with a large amount of water during the cutting process. After removing the enamel on the periphery of the cut transverse tooth slice, use extremely fine abrasive emery paper to polish the treated surface of the tooth slice under running water to make it smooth. Etch the treated surface of the dental sheet with 20% mass concentration of phosphoric acid for 60 seconds, rinse with enough deionized water to obtain a layer of about 5 μm thick complete demineralization (the apatite crystals between and within the fibers are dissolved, only Demineralized type I collagen fibers) are left in the dentin layer.

[0021] 1.2 Preparation of agar and solution

[0022] Prepare two groups of 100mL agar gels with a mass percentage...

Embodiment 2

[0029]The method for inducing the remineralization of tooth tissue, the steps are: preparation of dental slices: cut the collected teeth into thin slices perpendicular to the longitudinal axis of the teeth with a slow cutting machine, remove the enamel around the thin slices of teeth, and then polish them to be smooth, and use After phosphoric acid etched the tooth slices, rinse them with water to obtain a certain thickness of completely demineralized dentin layer; preparation of the mineralization system solution for inducing dentin remineralization: prepare two groups of agar gel with a mass percentage concentration of 0.5% One group is a blank gel; the other group of agar gel is added with phosphate, and the pH value of the gel is adjusted to 7.2 after heating and dissolving; prepare 0.13mol / L CaCl 2 Solution, pH=5~6; remineralization of dentin: put the acid-etched tooth thin slices in a centrifuge tube that is flattened with blank agar, first cover the tooth slices with pho...

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Abstract

The invention discloses a method for induction of remineralization of tissue of tooth, which comprises the following steps: preparing a dental film; preparing a dentin remineralization inducing mineralization system solution; and remineralizing the dentin. In the invention, the micro environment of the calcification of the tissue of the tooth in a gel matrix is stimulated, a calcium phosphate ion dispersing hydrogel system is constructed, and the biomimetic mineralization of the dentin is induced in vitro. The surface deposits are qualitatively characterized by constructing an agar gel mineralization system on the surface of the acid-corroded dentin in vitro and by suing a scanning electron microscope (SEM) and a micro-Fourier transform infrared spectrometer (FTIR). Results show an agar gel CaCl2 solution mineralization system containing phosphate can induce the deposition and growth of strip nano crystals on the surface of acid-corroded dentin surface, and that the deposited apatite crystals are densely arranged in an interlaced shape. The method is simple, convenient, quick and low in cost, and is an effective technical method for the biomimetic mineralization of the dentin.

Description

technical field [0001] The invention belongs to the technical field of bionic biomaterial engineering, and in particular relates to a method for inducing remineralization of tooth hard tissue in vitro without therapeutic purpose. Background technique [0002] Current research on dentin mineralization focuses on: resin-bonded hybrid layer (hybrid layer) demineralized dentin collagen fiber remineralization. The demineralized collagen fibers in the resin dentin bonded mixed layer are easily degraded by endogenous matrix metalloproteinases (MMP), which may increase microleakage and cause secondary caries, or cause the resin to come out and cause the failure of resin restoration. The issue of durability of resin-dentin-bonded restorations received a high level of attention in the 2009-2013 strategic plan of the National Institute of Dental and Craniofacial Research. A potential approach to improve the lifespan of dentin-resin bonds is to replace the residual moisture between dem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61C13/38
Inventor 刘来奎宁天云许小会朱丽芳叶金海杨聪翀夏露王培志
Owner NANJING MEDICAL UNIV