Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Formaldehyde biodegradation agent and preparation method thereof

A biodegradant, formaldehyde technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of consumption-dependent microorganisms and application limitations, and achieve good stability, short action time, and simple action conditions. Effect

Active Publication Date: 2011-09-14
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
View PDF2 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these microorganisms can only consume or degrade formaldehyde in the form of "in vivo", that is, the consumption of formaldehyde depends on the growth of microorganisms, and its application is greatly limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Formaldehyde biodegradation agent and preparation method thereof
  • Formaldehyde biodegradation agent and preparation method thereof
  • Formaldehyde biodegradation agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Formaldehyde biodegradation agent preparation embodiment

[0039] 1) After the strain Pseudomonas IOFA1 was activated with LB medium, it was inoculated in liquid LB medium at a ratio of 1%, and cultured with shaking at 30°C until OD 600 = 1.0;

[0040] 2) Add formaldehyde solution to the LB medium to a final concentration of 100mg / mL; continue shaking culture at 30°C for 36h;

[0041] 3) centrifuging the Pseudomonas IOFA1 culture solution in step 2) at 4°C, removing the supernatant, and collecting the cells;

[0042] 4) Add 200 mL of sterile water pre-cooled at 4°C to Pseudomonas IOFA1, suspend the bacteria, and collect the suspension;

[0043] 5) Break up the bacteria in the suspension, centrifuge at 4°C to remove the precipitate, filter the supernatant with a 0.22 μm filter membrane, and freeze-dry, the obtained dry powder is the formaldehyde biodegradant. The crushing can be crushed by a homogenizer, the conditions are: the homogenization pressure is ...

Embodiment 2

[0095] Similar to Example 1, the difference is that in step 1), OD 600 The value is 1.3; in step 2), after adding formaldehyde, the final concentration of formaldehyde in the culture medium is 150 mg / mL, and the culture time is 48 hours.

Embodiment 3

[0097] Similar to Example 1, the difference is that in step 1), OD 600 The value is 1.5; in step 2), after adding formaldehyde, the final concentration of formaldehyde in the culture medium is 200mg / mL, the temperature for continuing cultivation is 30°C, and the cultivation time is 60h.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a formaldehyde biodegradation agent and a preparation method thereof, relates to the biodegradation agent and provides a pseudomonad, namely IOFA1, as well as the formaldehyde biodegradation agent and the preparation method thereof. The formaldehyde biodegradation agent is dry powder of pseudomonad IOFA1 thallus breaking solution. The formaldehyde biodegradation agent adopts formaldehyde dehydrogenase as a main component. The preparation method comprises the following steps: activating the pseudomonad IOFA1 by using a culture medium, inoculating the pseudomonad IOFA1 into the culture medium, and performing shaking culture; adding formaldehyde solution into the culture medium, and continuing performing the shaking culture; performing centrifugation on culture solution at the temperature of 4 DEG C, removing supernatant liquid and collecting thalli; adding 100ml of sterile water into the thalli to suspend the thalli, and collecting suspension; and performing the centrifugation after breaking the thalli in the suspension, removing precipitate, filtering the supernatant liquid for removing bacteria, performing freeze-drying to get the dry powder of the pseudomonad IOFA1 thallus breaking solution, and further performing purification to get the formaldehyde biodegradation agent.

Description

technical field [0001] The invention relates to a biodegradation agent, in particular to a formaldehyde biodegradation agent and a preparation method thereof. Background technique [0002] Formaldehyde is a widely used important chemical raw material, but it is easy to cause air pollution and pose a great threat to people's health. Indoor air formaldehyde pollution mainly comes from various man-made panels for furniture and decoration. It is prone to addition, oxidation, reduction, and polymerization reactions. It has high water solubility and high reactivity of biological macromolecules. Formaldehyde is absorbed or degraded at the contact parts. After entering the human body, it mainly reacts with the protein and nucleic acid of the human body, damages the DNA, causes the gene mutation of the cell nucleus, cross-links within the DNA single strand, cross-links between DNA and protein, and inhibits the repair of DNA damage; combines with amino groups to change the interior of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N9/02B01D53/84B01D53/72A62D3/02A23L1/015C12R1/38A62D101/28A23L5/20
CPCY02A50/20
Inventor 曾润颖陈兴麟叶德赞
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com