Biological dye containing prodigiosins, preparation method thereof and application thereof
A prodigiosin and dye technology, applied in the fields of botanical equipment and methods, organic dyes, chemicals for biological control, etc. Problems such as poor presentability, to achieve the effects of good washing fastness and rubbing fastness, simple extraction process and good application prospects
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Embodiment 1
[0025] (1) Preparation and extraction of biochrome
[0026] Preparation of pigment by microbial fermentation process: culture medium formula: peptone 16g / L, glycerin 20g / L, MgSO 4 1.2g / L, NaCl 2.0g / L, Gly 2g / L, shake flask liquid volume 50mL / 250mL (V / V), inoculate Serratia marcescens jx-01 (CGMCC 4074) red pigment strain 5% (V / V ), pH 5.7, culture temperature 28°C, shaker speed 170r / min, culture 72h. Take the above fermented liquid and put it at 10000r·m -1 Centrifuge for 10min, discard the supernatant, add acetone to the sediment for 10min, and extract the extract at 10000r·m -1 After centrifuging for 10 min, the supernatant was rotary evaporated to constant weight, which was the red pigment solid of prodigiosin.
[0027] (2) Preparation of dye
[0028] Red pigment solid, diffusing agent sodium methylene bis-naphthalene sulfonate and zirconium beads with a diameter of 1.43mm are mixed in a mass ratio of 1:0.2:100 and put into a grinding cup to stir evenly, and then use a ...
Embodiment 2
[0048] The dyestuff prepared by the present invention dyes polyester fabric
[0049] Dyeing conditions: the amount of dye is 1% (o.w.f), the liquor ratio is 1:30, the dyeing temperature is 100°C, and the dyeing time is 45 minutes.
[0050] Dyeing process: pour water into the dye vat, heat to 50°C, weigh the dye, add a small amount of hydration material (to disperse the dye evenly in water), add to the dye vat, run for 2 minutes, add fabric, heat up to 80°C at 2°C / min, Reduce the heating rate, raise the temperature to 100°C at 1°C / min, dye for 40 minutes, release the residual liquid, wash twice with warm water, take it out, dehydrate, and dry.
[0051] (4) Dyeing effect
[0052] The color fastness and antibacterial properties of the dyed fabrics were tested, and the test results are shown in Table 4.
[0053] The test method of fastness to washing is based on GB / T3921·1-1997, and the rating is evaluated according to GB / T25O-1995; the determination of fastness to rubbing is ba...
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