Preparation method of yolk antibody sustained-release preparation for preventing gosling plague

A technology of egg yolk antibody and sustained-release preparation, which is applied in the field of preparation of egg-yolk antibody sustained-release preparation, can solve the problems of inconvenient storage, complicated process, short half-life and the like, and achieves the effects of scientific method, simple process and long storage period.

Inactive Publication Date: 2011-10-05
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By using the coating technology, the present invention solves the disadvantages of the existing gosling plague egg yolk antibody injection, such as inconvenient use, large side effects, complicated process, short half-life, and inconvenient storage.

Method used

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  • Preparation method of yolk antibody sustained-release preparation for preventing gosling plague
  • Preparation method of yolk antibody sustained-release preparation for preventing gosling plague

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Select 90-day-old healthy laying hens, and use gosling plague live vaccine for primary immunization (1ml intramuscular injection); at an interval of 7 days, use gosling plague oil emulsion inactivated vaccine for the second immunization (muscular injection 2ml); interval 14 The third immunization (3ml intramuscular injection) was carried out with the gosling plague oil emulsion inactivated vaccine; the fourth immunization (3ml intramuscular injection) was carried out with the gosling plague oil emulsion inactivated vaccine at an interval of 21 days. After the fourth immunization, the agar diffusion test was used to detect that the serum antibody titer reached 1:32, and the eggs laid were collected, and the yolk was separated aseptically. Mix egg yolk with an equal volume of 5% sodium chloride solution, add porous starch whose volume is 20% of the total volume of egg yolk and sodium chloride solution, and stir at 40°C for 60 minutes. Then the above liquid is mixed with s...

Embodiment 2

[0019] Select 90-day-old healthy laying hens, and use gosling plague live vaccine for primary immunization (1ml intramuscular injection); at an interval of 10 days, use gosling plague oil emulsion inactivated vaccine for the second immunization (3ml intramuscular injection); interval 21 On the second day, the third immunization (3ml intramuscular injection) was performed with the inactivated gosling plague oil emulsion vaccine. After the third immunization, the agar diffusion test was used to detect that the serum antibody titer reached 1:16, and the eggs laid were collected, and the yolk was separated aseptically. Mix the egg yolk with an equal volume of 5% sodium chloride solution, add porous starch whose volume is 30% of the total volume of the egg yolk and sodium chloride solution, and stir at 30°C for 50 minutes. Then mix the above liquid with an equal volume of sodium alginate solution with a mass concentration of 3%, and after fully mixing, dehydrate through spray dryin...

Embodiment 3

[0022] Select 150-day-old healthy breeding ducks and use gosling plague live vaccine for primary immunization (1ml intramuscularly); at an interval of 10 days, use gosling plague live vaccine for the second immunization (muscular injection 2ml); at an interval of 14 days, use Gosling plague oil emulsion inactivated vaccine for the third immunization (intramuscular injection of 3ml). After the third immunization, the agar diffusion test was used to detect that the serum antibody titer reached 1:64, and the eggs laid were collected, and the egg yolk was separated aseptically. Mix egg yolk with an equal volume of 5% sodium chloride solution, add porous starch whose volume is 40% of the total volume of egg yolk and sodium chloride solution, and stir at 30°C for 30 minutes. Then mix the above liquid with an equal volume of sodium alginate solution with a mass concentration of 4%, and after fully mixing, dehydrate through spray drying equipment to obtain a dry powder, which is the y...

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Abstract

The invention discloses a preparation method of a yolk antibody sustained-release preparation for preventing gosling plague, and belongs to the technical field of biological products. A healthy egg layer is immunized with a gosling plague live vaccine and a gosling plague inactivated vaccine successively; the laid egg is collected, and the yolk is separated; the yolk is well mixed with a sodium chloride solution, and a proper amount of porous starch is added for full adsorption; the above solution is well mixed with a sodium alginate solution; the mixed solution is dehydrated by a spray drying device; and a yolk antibody sustained-release preparation for preventing gosling plague is obtained. The method of the invention is scientific, has simple process, low cost, and minimal loss; the preparation has the characteristics of obvious efficacy, convenient preservation, good sustained-release performance, easy absorption, no toxicity, no residues, and the like. Compared with traditional yolk antibody injections, the yolk antibody sustained-release preparation for preventing gosling plague not only has a prolonged protective period, but also can be administered through an oral route without any stress response, and the preparation also has a certain growth-promoting effect.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a preparation method of an egg yolk antibody slow-release preparation for preventing and treating gosling plague. Background technique [0002] Gosling plague is a severe infectious disease caused by goose parvovirus, which mainly affects goslings and muscovy ducks. The morbidity and mortality are high, and the mortality rate of goslings or muscovy ducks can reach as high as 90%- 100%. The morbidity and mortality of gosling plague are closely related to the age of goslings (Muscos ducks). Under natural conditions, the disease only occurs in goslings (Muscos ducks) of 3-30 days old. [0003] For a long time, the prevention and control of gosling plague has been to use breeding geese (Muscos ducks) to be inoculated with gosling plague vaccine before laying, and to provide passive immunity to goslings (Muscos ducks) through maternal antibodies. However, due...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K9/14A61P31/20
CPCY02A50/30
Inventor 龚建森苗晋锋李新华倪同艳刘学贤吕晓娟李志兵
Owner JIANGSU INST OF POULTRY SCI
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