Exiguobacterium sp. with alga-lysing activity and application thereof in cyanobacterial bloom control
A microbacillus and algae-dissolving technology, which is applied in the direction of bacteria, microorganisms, biological water/sewage treatment, etc., can solve the problems of secondary pollution of water bodies, unsuitable water application, etc.
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[0020] 1. Screening of Alginolytic Bacteria
[0021] Inoculate 50 μl of natural water samples collected from Meiliangwan waters of Taihu Lake into 5mL LB liquid medium for enrichment for 24 hours (37°C, 200rpm fermentation culture), and add the culture solution to 100mL of Microcystis aeruginosa PCC7806 cultured to the logarithmic phase Algae liquid (algae cell density about 1×10 7 individual / mL). Two days later, take the yellow algae liquid and spread it on LB plate by gradient dilution method, incubate at 37°C for 24 hours, take a plate with moderate colony density, and select different strains according to the different colony forms.
[0022] The selected algae-lytic bacteria were respectively inoculated into 10 mL of LB medium according to the ratio of 1%, cultivated at 37 ° C for 24 hours at 200 rpm, and 10 mL of the cultured bacteria liquid was added to 90 mL of the algae liquid of Microcystis aeruginosa in the logarithmic phase ( The algae cell density is about 1×10 ...
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