Exiguobacterium sp. with alga-lysing activity and application thereof in cyanobacterial bloom control

A microbacillus and algae-dissolving technology, which is applied in the direction of bacteria, microorganisms, biological water/sewage treatment, etc., can solve the problems of secondary pollution of water bodies, unsuitable water application, etc.

Inactive Publication Date: 2011-10-05
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

The methods of chemical algae removal mainly include the application of herbicides, algicides and metal salts, etc. Although the chemical algae removal techn...

Method used

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  • Exiguobacterium sp. with alga-lysing activity and application thereof in cyanobacterial bloom control
  • Exiguobacterium sp. with alga-lysing activity and application thereof in cyanobacterial bloom control
  • Exiguobacterium sp. with alga-lysing activity and application thereof in cyanobacterial bloom control

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Embodiment

[0020] 1. Screening of Alginolytic Bacteria

[0021] Inoculate 50 μl of natural water samples collected from Meiliangwan waters of Taihu Lake into 5mL LB liquid medium for enrichment for 24 hours (37°C, 200rpm fermentation culture), and add the culture solution to 100mL of Microcystis aeruginosa PCC7806 cultured to the logarithmic phase Algae liquid (algae cell density about 1×10 7 individual / mL). Two days later, take the yellow algae liquid and spread it on LB plate by gradient dilution method, incubate at 37°C for 24 hours, take a plate with moderate colony density, and select different strains according to the different colony forms.

[0022] The selected algae-lytic bacteria were respectively inoculated into 10 mL of LB medium according to the ratio of 1%, cultivated at 37 ° C for 24 hours at 200 rpm, and 10 mL of the cultured bacteria liquid was added to 90 mL of the algae liquid of Microcystis aeruginosa in the logarithmic phase ( The algae cell density is about 1×10 ...

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Abstract

The invention discloses Exiguobacterium sp. with alga-lysing activity and application thereof in cyanobacterial bloom control and belongs to the technical field of microbe application. The Exiguobacterium sp. A27 with higher alga-lysing activity is separated from water of Taihu Lake, and has the collection number of GCMCC (China General Microbiological Culture Collection Center) No.4114. The Exiguobacterium sp. A27 is used for development and production of novel algicides, and is finally applied to controlling cyanobacterial bloom in lakes.

Description

technical field [0001] The invention relates to a bacterial strain and a method in the technical field of microbial application, in particular to a microbacterium with algicidal activity and its application in the control of cyanobacteria blooms. Background technique [0002] With the intensification of eutrophication in global water bodies, the outbreak of harmful algal blooms is becoming more and more frequent, and the environmental and economic problems caused by it have attracted people's attention. When source and non-point source pollution control requires huge investment and is difficult to achieve results in the short term, it is very necessary to explore effective ways to control phytoplankton biomass and inhibit the occurrence of "algae blooms". Algae control techniques can be classified into physical methods, chemical methods and biological methods. Physical methods are mainly electromagnetic fields, mechanical algae removal, etc., which can be used as auxiliary ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 杨虹田川潘建良谭晶李道棠
Owner SHANGHAI JIAO TONG UNIV
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