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Method for increasing yield of Cordycepin

A cordycepin, content technology, applied in the biological field, can solve the problems of low yield and application limitations, and achieve the effects of reliable source, easy cultivation and preservation, and stable conditions

Inactive Publication Date: 2011-10-19
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cordycepin ( cordycepin ) is a class of nucleoside antibacterial substances isolated from Cordyceps militaris, which has a variety of biological activities, but cordycepin is a secondary metabolite, and the yield is not high, so its application is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for improving the content of cordycepin, comprising the following steps:

[0022] (1) Preparation of PDA slant medium

[0023] Cut 200g of potatoes into cubes, boil in water for 30min, filter to get the juice, add 20g of glucose, 20g of agar, make up 1000mL of water, natural pH; sterilization conditions: 121°C, 30min;

[0024] (2) Preparation of PDA liquid seed medium

[0025] Cut 200g of potatoes into pieces, boil in water for 30min, filter to get the juice, add 20g of glucose, make up to 1000mL of water, the pH is natural; sterilization conditions: 121°C, 30min;

[0026] (3) Preparation of PDA fermentation medium

[0027] Cut 200g of potatoes into pieces, boil in water for 30min, filter to get the juice, add 20g of glucose, make up to 1000mL of water, the pH is natural; sterilization conditions: 121°C, 30min;

[0028] (4) Preparation of spore suspension

[0029] Paecilomyces pupae ( Paecilomyces militaris ) The 08-01 strain was inoculated in PDA slant m...

Embodiment 2

[0035] A method for improving the content of cordycepin, comprising the following steps:

[0036] (1) Preparation of PDA slant medium

[0037] (2) Preparation of PDA liquid seed medium

[0038] (3) Preparation of PDA fermentation medium

[0039] (4) Preparation of spore suspension

[0040] (5) Preparation of liquid stock

[0041] Above five steps are with embodiment 1;

[0042] (6) Vaccination:

[0043] Inoculate into PDA fermentation medium with 4% (v / v) inoculum, add 7% oleic acid at the same time, 140rpm, 26°C, shake flask culture for 7 days. The total amount of cordycepin obtained was 5.608±0.01 mg / L, which was 2.52 higher than that of the fermentation system without oleic acid.

Embodiment 3

[0045] A method for improving the content of cordycepin, comprising the following steps:

[0046] (1) Preparation of PDA slant medium

[0047] (2) Preparation of PDA liquid seed medium

[0048] (3) Preparation of PDA fermentation medium

[0049] (4) Preparation of spore suspension

[0050] (5) Preparation of liquid stock

[0051] Above five steps are with embodiment 1;

[0052] (6) Vaccination:

[0053] Inoculated into the PDA fermentation medium with 4% (v / v) inoculum, and added 8% oleic acid at the same time, 140rpm, 26°C, and cultured in shake flasks for 7 days. The total amount of cordycepin obtained was 5.621±0.01mg / L, which was 2.53% higher than that of the fermentation system without oleic acid.

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PUM

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Abstract

The invention discloses a method for increasing content of Cordycepin, which comprises the following steps: inoculating Paecilomyces miliraris 08-01 to a PDA (potato dextrose agar) slant culture medium, culturing at 26 DEG C for 5 days, activating three times, inoculating into the PDA liquid seed culture medium, carrying out shake culture at 140rpm at the temperature of 26 DEG C for 3 days to obtain a liquid seed, inoculating into the PDA fermentation culture medium (the inoculum size is 4%(V / V)), adding organic solvent oleic acid (v / v is 6-8%) at the same time, and carrying out shake culture at 140rpm at the temperature of 26 DEG C for 7 days to obtain the Cordycepin. The method has the advantages of obviously increased Cordycepin content, accessible culture medium and stable and simple fermentation conditions.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for increasing the yield of cordycepin. Background technique [0002] Two-phase culture, also known as two-phase culture, two-phase fermentation, two-phase fermentation, cell culture-separation coupling process. By adding water-soluble or fat-soluble organic substances, or polymeric compounds with adsorption effect, to plant cells or microbial culture, the culture system forms two phases due to the difference in distribution coefficient, and the cells grow in one of the phases. Secondary metabolites are synthesized, and these products are released outside the cell through active or passive transport, and are adsorbed by another phase. Two-phase culture greatly reduces the inhibitory effect of the product itself on cell metabolism, increases the yield of metabolites, and protects the product from the influence of catalytic enzymes or acids in the medium on the product. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40C12R1/79
Inventor 李祝刘吴娟任秀秀肖洋陈青周礼红
Owner GUIZHOU UNIV
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