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Preparation method of trichoderma longibrachiatum lyase for treating fungal diseases

A technology of Trichoderma longifolia and lyase, which is applied in the field of preparation of Trichoderma longifolia lyase, can solve the problems of fungal disease research and literature reports, public disclosure, and no preparation method without the lyase of Trichoderma longifolia. Achieve the effect of high product purity, low fermentation cost and high enzyme activity

Inactive Publication Date: 2011-11-02
GUANGZHOU MINGSHIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there are examples of using lysozyme as a drug for treating bacterial diseases at home and abroad, but there is no research and literature report on the use of lysozyme as a treatment for fungal diseases, and there is no relevant disclosure of literature about its preparation method.

Method used

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  • Preparation method of trichoderma longibrachiatum lyase for treating fungal diseases
  • Preparation method of trichoderma longibrachiatum lyase for treating fungal diseases
  • Preparation method of trichoderma longibrachiatum lyase for treating fungal diseases

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Embodiment 1

[0029] A. The formula of the fermentation medium for culturing Trichoderma longibrachiatum to produce Trichoderma longibrachia lyase is as follows:

[0030] Sugarcane bran 3%, drum skin 1.0%, ammonium sulfate 1.5%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.5%, agar 1.0%, trace elements 0.05%, the rest is deionized water, in weight percentage.

[0031] B. The method of preparing Trichoderma longibrachia lyase using the above fermentation medium is as follows:

[0032] Prepare 20L fermentation broth (using deionized water) in the fermentation tank according to the above formula, and take the Trichoderma longibrachiatum strain. The longibrachiatum enzyme strain is purchased from Guangdong Institute of Microbiology and is Trichoderma Longibrachiatum Rifai. Strain 958-11 was obtained by selection and breeding. After sterilization, the inoculation of the bacteria is 5% of the volume of the fermentation broth, and the bacteria concentration is 15%. The bacteria concentration ...

Embodiment 2

[0042] A. The formula of the fermentation medium for culturing Trichoderma longibrachiatum to produce Trichoderma longibrachia lyase is as follows:

[0043] Sugarcane bran 5%, drum skin 2.0%, ammonium sulfate 2.5%, potassium dihydrogen phosphate 1.5%, magnesium sulfate 1.5%, agar 2.0%, trace elements 0.3%, the rest is deionized water, calculated by weight percentage.

[0044] B. The method of preparing Trichoderma longibrachia lyase using the above fermentation medium is as follows:

[0045] Prepare 20L fermentation broth (using deionized water) according to the above formula and place it in a fermenter. Take the Trichoderma longibrachiatum strain. The strain of Trichoderma longibrachiatum Rifai is provided by the Guangdong Institute of Microbiology and is Trichoderma Longibrachiatum Rifai. Strain 958-11 was selected and obtained. After sterilization, the strain was inoculated. The inoculation amount of the strain was 10% of the volume of the fermentation broth and the bacterial conc...

Embodiment 3

[0055] A. The formula of the fermentation medium used to cultivate Trichoderma longibrachiatum to produce Trichoderma longibrachia lyase is as follows:

[0056] Sugarcane bran 8%, drum skin 5.5%, ammonium sulfate 4%, potassium dihydrogen phosphate 2%, magnesium sulfate 2.5%, agar 3.0%, trace element 0.5%, the rest is deionized water, in weight percentage.

[0057] B. The method for preparing Trichoderma longibrachia lyase using the above fermentation medium is as follows:

[0058] Prepare 20L of fermentation broth (using deionized water) according to the above formula and place it in a fermenter, and take the Trichoderma longibrachiatum strain, among which the Trichoderma Longibrachiatum Rifai strain is provided by Guangdong Institute of Microbiology. Strain 958-11 was obtained by breeding. After sterilization, the strain was inoculated. The inoculation amount of the strain was 15% of the volume of the fermentation broth and the bacterial concentration was 25%. The bacterial concentr...

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Abstract

The invention discloses a preparation method of trichoderma longibrachiatum lyase for treating fungal diseases. The preparation method provided by the invention comprises the following steps of: preparing a fermenting culture medium of the trichoderma longibrachiatum and adding trichoderma longibrachiatum strains into the fermenting culture medium; adjusting the temperature to 25-30 degrees centigrade, adjusting the pH value to 6.0-7.5 and adjusting the dissolved oxygen to 40-55%; and fermenting for 3-6 days to obtain fermenting liquid of the trichoderma longibrachiatum; separating and purifying the obtained trichoderma longibrachiatum fermenting liquid to obtain trichoderma longibrachiatum lyase. The target enzyme which is obtained by the method provided by the invention has the advantages of high strain yield, high target enzyme expression amount and high purity and enzyme activity; the method provided by the invention has the advantages of short fermenting time, low fermenting cost and precise separating process; and the trichoderma longibrachiatum lyase which is obtained by utilizing the method provided by the invention has a stronger anti-fungal function and can be used as an anti-fungal medicine to be developed and utilized.

Description

technical field [0001] The invention relates to a preparation method of a lyase, in particular to a preparation method of a Trichoderma longanum lyase for treating fungal diseases. Background technique [0002] At present, there are examples of using lysozyme as a drug for treating bacterial diseases at home and abroad, but there is no research and literature report on the use of lysozyme as a treatment for fungal diseases, and there is no relevant disclosure of literature about its preparation method. . Contents of the invention [0003] The object of the present invention is to provide a kind of preparation method of the Trichoderma longifolia lyase that is used for the treatment of fungal disease, and this method fermentation time is short, and cost is low; The yield of the target enzyme prepared by adopting this method is high, and target enzyme expression amount High and the purity and enzymatic activity of the target enzyme are high. [0004] The first object of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88A61K38/51A61P31/10C12R1/885
Inventor 安鸿尹海滨
Owner GUANGZHOU MINGSHIN PHARMA
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