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Tissue-culture quick breeding method for iris tectorum

A tissue culture rapid propagation technology, applied in the field of plant tissue culture, can solve the problems of high explant contamination rate and low bud induction rate, and achieve the effect of realizing large-scale production, increasing the multiplication coefficient and the strong seedling rate

Inactive Publication Date: 2012-11-14
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of iris group with simple operation, low pollution rate, high plant regeneration efficiency and high seedling quality for the defects of high explant contamination rate and low bud induction rate in the existing iris tissue culture technology. Rapid Propagation Method

Method used

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  • Tissue-culture quick breeding method for iris tectorum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: (a kind of tissue culture method 1 of Iris)

[0036] Follow these steps:

[0037] (1) The preparation of the culture medium, including each component of the basic culture medium and the culture medium of each stage of tissue culture and the weight per liter:

[0038] 1) Basic medium: LS medium, wherein, sugar 20g / L, agar 5.5g / L, pH5.8;

[0039] 2) Bud induction medium: LS+ Shannong No. 1 7ml / L+6-BA0.5mg / L+NAA0.2mg / L;

[0040] 3) Subculture proliferation medium: LS+6-BA1.0mg / L+NAA0.3mg / L;

[0041] 4) Strong seedling medium: LS+6-BA0.3mg / L and NAA0.5mg / L;

[0042] 5) Rooting medium: LS+NAA1.0mg / L+GA0.3mg / L;

[0043] Wherein the formula of LS basic medium is;

[0044]

[0045] (2) The cultivation of iris virus-free tissue culture seedlings:

[0046] 1) Selection and sterilization of explants: Take the young underground buds of healthy iris plants, cut off the leaves and roots above the base, soak in 10% Liby detergent essence for 8-10 minutes, rinse ...

Embodiment 2

[0054] Embodiment 2: (a kind of tissue culture method 2 of Iris)

[0055] In this example, the preparation of step (1) medium: white sugar 15g / L, agar 6g / L, pH5.7 in the basic medium; bud induction medium is: LS+Shannong No. 1 5ml / L+6-BA0 .3mg / L+NAA 0.1mg / L; Subculture medium: LS+6-BA0.5mg / L+NAA0.2mg / L; Strong seedling medium: LS+6-BA0.3mg / L+ NAA 0.6mg / L; rooting medium: LS+NAA0.8+GA 3 0.4mg / L; Step (2) Cultivation of Iris detoxified tissue-cultured seedlings: 1) Selection and sterilization of explants: Young shoots were soaked in 70% alcohol for 30s, soaked in 0.1% mercuric chloride aqueous solution for 8min, and then removed Stem tip tissue block and cut into 0.4cm 3 Small block; the light intensity of each stage of bud induction, subculture multiplication, strong seedlings and rooting culture is 2500Lx; all the other steps are the same as in Example 1.

Embodiment 3

[0056] Embodiment 3: (a kind of tissue culture method 3 of Louisiana Iris "cherry")

[0057] In this example, the preparation of step (1) medium: white sugar 25g / L, agar 7g / L, pH5.6 in the basic medium; bud induction medium is: LS+Shannong No. 1 8ml / L+6-BA0 .8mg / L+NAA 0.2mg / L; subculture medium: LS+6-BA0.8mg / L+NAA0.4mg / L; strong seedling medium: LS+6-BA0.4mg / L+ NAA 0.7mg / L; rooting medium: LS+NAA0.5+GA 3 0.5mg / L; Step (2) Cultivation of Iris detoxified tissue-cultured seedlings: 1) Selection and sterilization of explants: Young shoots were soaked in 70% alcohol for 50s, soaked in 0.1% mercuric chloride aqueous solution for 12min, and then removed Stem tip tissue chunks and cut into 0.45cm 3 Small block; the light intensity of each stage of bud induction, subculture multiplication, strong seedlings and rooting culture is 1800Lx; all the other steps are the same as in Example 1.

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Abstract

The invention discloses a tissue-culture quick breeding method for iris tectorum, belonging to the technical field of plant tissue culture. The method comprises the following technical steps: (1) preparing a culture medium; (2) culturing iris tectorum detoxified tissue-culture seedlings; and (3) hardening and transplanting the tissue-culture seedlings. With the method, through adjustment on the type and the content of culture medium hormones, addition of antibiotics and other technical measures, the pollution rate of a iris tectorum explant is obviously lowered, and an inoculation survival rate is above 90%; the induction differentiation rate of the explant can be obviously improved to 99% from 70-80% in the prior art; and the tissue-culture strong seedling rate of the iris tectorum is improved. The tissue-culture quick breeding method can be popularized and applied to landscaping and tissue culture enterprises.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of Iris Louisiana. Background technique [0002] Louisiana Iris is an aquatic flower plant of the Iridaceae family Iris, native to Louisiana, USA. This plant has already been widely used in some countries in North America and Europe. Its flowering period is from April to May, and its flowers are rich in color. , strong drought tolerance, and extensive management tolerance. At the same time, it has the advantages of evergreen in the south of the Yangtze River in my country. The current market situation is optimistic, but high-quality seedlings are in short supply. In the past, this series of flowers were mainly propagated by ramets and seeds, which not only slowed the propagation speed, was labor-intensive and time-consuming, and could not meet the needs of the market; at the same time, the seed germination rate of this kind of plants was ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘慧春朱开元周江华邹清成马广莹
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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