Phellinus igniarius fermentation medium

A fermentation medium, P. baumannii technology, applied in fungi, horticulture, botanical equipment and methods, etc., can solve the problem of no clear medium formula, etc., and achieve strong knot holding force, high inoculation survival rate, The effect of uniform pore size distribution

Inactive Publication Date: 2007-09-19
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The artificial cultivation of Phellinus baumannii is still in its infancy, and at present only South Korea has reported that the fruiting body of this bacterium has been successfully cultivated, Song et al., (Song CH, Moon H H, Ryu CH, 1997.Artificial cultivation of Phellinus linteus.Korean J Mycol: 130~132), but there is no clear medium formula
At present, there is no successful artificial culture report in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) The fruiting bodies of the wild Pharonotus baumannii grown in Syringa amurensis Rupr. were isolated and further purified.

[0021] 2) Expansion of the mother species: the original species of the mother species of Phylophorus baumannii is cultivated according to a conventional method, and the culture medium adopted is a conventional PDA medium or a PSA medium,

[0022] The weight composition of PDA medium is, water: potato: agar: glucose: KH 2 PO 4 :MgSO 4 : VB 1 =2000:400:40:40:6:3:0.02; pH value 5.5;

[0023] The weight composition of PSA medium is water: potato: agar: sucrose: KH 2PO 4 : MgSO 4 : VB 1 =2000:400:40:40:6:3:0.02; pH value 6.5;

[0024] The above two formulas can be used as the culture medium for strain isolation, and can also be used as the culture medium for the propagation of the mother species.

[0025] a. The formula of the PDA mother seed culture medium adopted in the present embodiment: 200g of potatoes, 20g of glucose, 20g of agar, 100...

Embodiment 2

[0046] The difference from Example 1 is that the PSA medium is used in the multiplication of the female species, which consists of water: potato: agar: sucrose: KH 2 PO 4 : MgSO 4 : VB 1 =2000:400:40:40:6:3:0.02; pH value 6.5; medium sterilization pressure: 1.2kg / cm 2 , Sterilization time: 30 minutes, Sterilization temperature: 121°C;

[0047] The formula of the culture medium for the fermentation culture of Phylophorus baaumannii (original species culture and cultivated species culture) is: Paomazi clove sawdust (dry weight) 975~1025Kg, corn flour 120~125Kg, gypsum 14~16Kg, KH 2 PO 4 2.75-3.25Kg, mannitol 9.5-10.5Kg, beef extract 9.5-10.5Kg.

Embodiment 3

[0049] The difference from Example 1 is that: the medium formula of the Fermentation Culture of Phylophorus baumannii (Cultivation of Original Species and Cultivation of Cultivars) is: Chestnut Sawdust (Dry Weight) 975~1025Kg, Corn Flour or Sorghum Rice Flour 120~125Kg, Gypsum 14~16Kg, KH 2 PO 4 2.75-3.25Kg, 9.5-10.5Kg of sucrose or glucose, 9.5-10.5Kg of ammonium sulfate.

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PUM

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Abstract

The invention provides a culture medium for Phellinus igniarius, which comprises the following constituents (by weight percentage): 1950-2050 parts of Syringa amarensis Rupr. wood flour, Chinese chestnut or pear wood flour, 240-260 parts of maize flour, Chinese sorghum bran and / or Chinese sorghum flour, 23-32 parts of gypsum, 5.5-6.5 parts of KH2PO4, 19-21 parts of ammonium sulfate, sucrose or glucose, or 19-21 parts of mannitol and beef grease.

Description

technical field [0001] The invention relates to the artificial cultivation of Pharonotus baumannii, in particular to a fermentation medium for Pharonotus boaumannii. Background technique [0002] Phomalofora baumannii is known to have the best anti-cancer effect among higher fungi, and its market development prospect is very optimistic. [0003] The anticancer function of Phellinus basidiomycetes was first discovered by Japanese scholars Tetsuro Ikekawa et al. 155~157) found that the study showed that the extract of the wild fruiting body of S. baumannii had an inhibitory rate of 96.7% on mouse sarcoma 180; the study also showed that the substance with anticancer effect was the polysaccharide in the fruiting body of S. baumannii . Naruse et al. (Naruse S, Takeda S, Ito H, Jujii K, Terada Y, Shimura K, Sugiura M and Miyazaki T, 1974, Studies on antitumor activity of basidiomycetes: 2.Antitumor effects of polysaccharides prepared from cultured basidiomycetes. Mie Med J, 23 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G1/04
Inventor 戴玉成刘春静杜佳艳魏玉莲
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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