Bacillus pumilus with anti-mildew function

A Bacillus pumilus and anti-mold technology, which is applied in the direction of bacteria, application, and microbial-based methods, can solve the problem that there is no effective method for preventing mildew in stored cigarettes, so as to protect health, have strong anti-mold activity, and reduce economic losses. Effect

Inactive Publication Date: 2013-09-25
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no effective way to prevent mildew in stored tobacco. In production, most of them avoid or reduce the loss of mildew in tobacco by strengthening the management of the storage environment (such as adjusting the temperature and humidity of the environment).
The inventor of the present invention, on the basis of systematic research on the microbial flora on the surface of tobacco, screened and obtained excellent strains with anti-mold effect and improved the quality of tobacco, but none of the patent documents that have been applied for relates to the subject of the present invention literature reports

Method used

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  • Bacillus pumilus with anti-mildew function
  • Bacillus pumilus with anti-mildew function
  • Bacillus pumilus with anti-mildew function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Biocontrol Bacteria

[0029] Accurately weigh 10.0 g of the sheet tobacco sample, put it into a conical flask with 100 mL of sterile water, shake it on a shaker at 200 rpm / min for 30 min, filter it with a single layer of gauze, and dilute the eluate 4 to 5 times in a 10-fold series, and use The micropipette draws 10 in turn from low concentration to high concentration -6 、10 -5 、10 -4 0.1 mL each, spread on beef extract peptone medium, repeat each dilution three times, and culture at 28°C for 48-72 hours. The appropriate dilution is 50-100 single colonies per plate. Pick different types of colonies and purify them on the NA plate for 3 times, then streak on the NA slant, and store at 4°C for future use. Inoculate 3 bacterial strains near the edge of the NA medium plate, culture at 25°C for 24 hours, after the bacterial band grows, inoculate the mold block in the center of the plate Take the plate only inoculated with moldy ...

Embodiment 2

[0033] The influence of embodiment 2 biocontrol bacterial strains on the fungal fungi

[0034] Weigh 5.0 g of tobacco flakes, put them into a conical flask, and sterilize at 121°C for 20 minutes. Mix the bacterial suspension and the mold spore liquid at 0:1 (CK2), 1:20 (v / v), 1:40, and 1:80 respectively, then use a medical inoculator to spray 1.0 mL of the spore mixture, and place it at 28 Cultivate at 85% relative air humidity, record the date of mildew of tobacco leaves, and determine the control effect of biocontrol strains on mildew of tobacco leaves. Inoculate sterile water as a control (CK1). The results are shown in Table 2. The mixed inoculation of bacterial strains and mold spores in tobacco has a certain inhibitory effect on the growth of mold on tobacco, and can delay the time and severity of mold in tobacco.

[0035] Table 2 The influence of LZ-7 on the fungigenicity of some molds

[0036]

[0037]*The degree of mildew refers to the following criteria: no mil...

Embodiment 3

[0038] Embodiment 3 biocontrol fungicide is to the indoor test of laminar tobacco antimold

[0039] Dilute the LZ-7 strain bacterial suspension to 20 times, 40 times and 80 times respectively, spray 20mL to 100g sheet tobacco with a medical inoculator respectively (the moisture content of the sheet tobacco is about 32%), seal the plastic bag, and use aseptic Water was used as a control, and each treatment was repeated 3 times, cultured at 28°C, and the date of tobacco leaf mildew was recorded to determine the control effect of the biocontrol strains on tobacco leaf mildew. The results are shown in Table 3. LZ-7 has an effective With a certain control effect, it can significantly delay the occurrence time of tobacco mildew. LZ-7 strain can delay the mildew of tobacco for 7 days when diluted 80 times, and it can delay the mildew of tobacco for 15 days when diluted 40 times. It can delay the mold of tobacco for 15 days when diluted 20 times, and it can delay the mildew of tobacco ...

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Abstract

The invention discloses a bacterial strain for preventing mildew of stored flue-cured tobacco strips, and belongs to the field of biological control of plant diseases. The strain has been collected in China General Microbiological Culture Collection Center on August 23, 2010 with the collection number of CGMCC No.4116, and is classified and named Bacillus pumilus. The strain can inhibit the growth of mould of the flue-cured tobacco strips, has good control effect on the mildew caused by the mould, and comprises an active ingredient of a whole culture solution culture or spores of the Bacilluspumilus LZ-7. The bacterial strain has the advantages of low production and use cost, convenience in use, good anti-mildew effect, promotion of alcoholization of the flue-cured tobacco strips and thelike, and overcomes the defects that a chemical mildewproof agent easily influences the quality of the flue-cured tobacco strips, easily causes residue and the like.

Description

technical field [0001] The invention provides a bacillus pumilus strain which has anti-mold effect on stored tobacco and its application, belonging to the field of biological control. Background technique [0002] The natural alcoholization or artificial fermentation of tobacco leaves is one of the necessary steps to improve the smoking quality and usability of tobacco leaves. The alcoholic storage period of sheet tobacco is as long as 1-2 years, during which mildew caused by microorganisms is very likely to occur, which reduces the quality of tobacco leaves or completely loses their use value. Spoilage is the softening and odor deterioration caused by the growth and reproduction of bacteria. Mildew refers to the deterioration caused by mold, which accounts for a large proportion of the storage loss of tobacco leaves, seriously affects the appearance quality and aroma quality of tobacco leaves, and reduces the use value of tobacco leaves. It is one of the important safety i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/07
Inventor 黄建张成省孔凡玉王凤龙王静冯超马强林建胜周显升王新伟蒋光伟
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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