An expression vector suitable for three host systems

Abstract

The invention discloses a carrier plasmid capable of expressing proteins in three host systems, including Escherichia coli, insect cells and mammalian cells. The expression vector (plasmid) contains three kinds of promoters suitable for transcription initiation of different vector systems, and contains transposable elements to facilitate site-directed transfer of the expression framework to the Bacmid shuttle plasmid. The plasmid can be applied to expression of recombinant protein, rapid selection of suitable expression host, and subsequent mass expression and preparation of recombinant protein.

Description

technical field [0001] The invention relates to recombinant protein expression technology, in particular to an expression vector, in particular to an expression vector applicable to three host systems of Escherichia coli, insect cells and mammalian cells. Background technique [0002] With the rapid development of research and industry in the field of biomedicine, there is an increasing demand for recombinant proteins. The expression amount, purification difficulty and activity of different recombinant proteins in different expression hosts are very different. In order to obtain highly expressed and highly active recombinant proteins, it is often necessary to try different expression systems. Due to the different expression elements (such as promoters) used in different expression systems, it usually needs to be cloned into a host-specific expression vector when trying, which is relatively high in workload and cost, and takes a long time. Therefore, a vector suitable for m...

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Problems solved by technology

Due to the different expression elements (such as promoters, etc.) used in different expression systems, it is usually necessary to clone into a host-specific expression vector when trying, which is relatively high in workload and cost, and takes a long time

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