Method for improving biological control efficacy of pichia caribbica to fruit postharvest diseases

A technology for postharvest diseases of fruits and Pichia pastoris, which is applied in the field of postharvest biological control of fruits, can solve problems such as affecting the use of antagonistic bacteria, and achieve the effects of inhibiting fruit pathogenic bacteria, having strong antagonistic effect and preventing rot.

Inactive Publication Date: 2011-12-21
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These factors have affected the use of antagonistic bacteri

Method used

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  • Method for improving biological control efficacy of pichia caribbica to fruit postharvest diseases
  • Method for improving biological control efficacy of pichia caribbica to fruit postharvest diseases
  • Method for improving biological control efficacy of pichia caribbica to fruit postharvest diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Inhibitory effect of trehalose-induced cultivation of Pichia pastoris on fruit diseases

[0038] 1. Test plan

[0039] After the fruit has been processed, wounds of uniform size and depth are formed on the equatorial part of the surface of each fruit with a sterile punch. Among them, the surface wound of strawberry is 3mm (diameter)×3mm (depth), the surface wound of peach fruit is 5mm (diameter)×3mm (depth), and the surface wound of apple is 5mm (diameter)×5mm (depth). Add 30 μl of (1) yeast suspension cultured in NYDB medium; (2) yeast suspension cultured in trehalose-supplemented medium (trehalose and glucose for carbon source) into each wound; (3) sterile water (as a control). After 3h, an equal amount of 30 μl of pathogenic spores was added to each wound ( B. cinerea The concentration is 1×10 5 spores ml -1 ; P. expansum The concentration is 5 x 10 4 spores / mL, R. stolonifer The concentration is 1×10 4 spores / mL) (spores / mL means the numb...

Embodiment 2

[0048] Example 2: Effect of trehalose-induced cultivation of Pichia pastoris on fruit storage quality

[0049] 1. Test plan

[0050] After the test fruit was picked, soak the fruit for 1 min directly with the following treatments: (1) Pichia pastoris suspension cultured in NYDB medium; Yeast suspension; (3) Sterile water (as a control). Then put the fruit in a plastic basket, seal it with plastic wrap after air drying, and cultivate it in a constant temperature incubator (humidity 95%). Put strawberries and apples at 20°C, and analyze the storage quality after a period of time. Each treatment was repeated 3 times, each time 10 fruits were repeated, and the whole experiment was repeated 2 times.

[0051] The storage quality analysis method is as follows:

[0052] (1) Weight loss rate: weighing method. Weight loss rate = (mass before treatment - mass after storage) / mass before treatment × 100%

[0053] (2) Hardness: Tested with TA-XT2i texture analyzer, the diameter of ...

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Abstract

The invention discloses a method for improving biological control efficacy of pichia caribbica to fruit postharvest diseases, belonging to the field of fruit postharvest biological control. The method comprises the steps of: activating pichia caribbica, inoculating in an NYTB culture medium added with 0.2-1% of trehalose for inducing culture, and centrifuging to obtain thalli; diluting the thalli with sterile water to prepare 1*10<8>/ml of bacterial suspension; placing fruits in the bacterial suspension, soaking for 1 minute and then immediately taking out, and naturally airing; and placing in a plastic crate, sealing with a preservative film, and storing in an environment at room temperature. The efficacy of controlling the fruit postharvest diseases by the pichia caribbica is increased by using the inducing culture of the trehalose, thus the method has the advantages of simplicity in use, convenience for operation, good effect and low cost. The pichia caribbica subjected to the inducing culture can be used for replacing a chemical bactericide to control the fruit postharvest diseases, can avoid the damage to people due to the use of the chemical bactericide, and has remarkable economic benefit and social benefit.

Description

technical field [0001] The present invention relates to a method of using trehalose (trehalose) to induce culture to improve the strain of Pichia pastoris ( Pichia caribbica ) is a method for preventing and controlling fruit postharvest diseases, and belongs to the field of fruit postharvest biological control. Background technique [0002] The loss caused by rot in the process of fruit picking, transportation and storage is huge, and it is also a problem of global concern. According to reports, in developed countries, 10%-30% of fresh products are rotted due to post-harvest diseases, while in developing countries that lack storage and transportation refrigeration equipment, the rate of post-harvest fruit rot is as high as 40%-50%. Foreign fruit storage and freshness accounts for more than 60% of the total fruit output. At present, the fruit storage capacity in my country is more than 8 million tons, which is less than 15% of the total. Among them, the storage capacity of c...

Claims

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Application Information

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IPC IPC(8): A23B7/155C12N1/16C12R1/84
Inventor 张红印赵利娜任晓锋张晓云刘伟民朱淑云代春华
Owner JIANGSU UNIV
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