Preparation method of nontoxic cotton dreg animal feed
A technology of animal feed and cotton meal, which is applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the problems of large investment and high corrosion resistance requirements, and achieve the goals of wide application, increased feed intake, and accelerated fermentation speed Effect
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Embodiment 1
[0032] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:
[0033] 1) Preparation of primary strains:
[0034] a. Use an inoculation loop to inoculate a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae into a 250mL Erlenmeyer flask filled with 100mL liquid yeast culture medium, shake at 30°C Cultivate for 36 hours until the concentration of each bacterial solution is 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;
[0035] in:
[0036] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 7.0.
[0037] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 9g / L, beef extract 5.5g / L, glucose 11g / L, sodium chloride 4.5g / L, Ph 6.5.
[0038] The concentration of e...
Embodiment 2
[0051] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:
[0052] 1) Preparation of primary strains:
[0053] a. Using an inoculation loop, pick a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae respectively and inoculate them into 250mL Erlenmeyer flask yeast culture medium filled with 100mL liquid yeast culture medium. Shake culture at 30°C for 36 hours until the concentration of each bacterial solution reaches 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;
[0054] in:
[0055] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 6.5.
[0056] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 10g / L, beef extract 5g / L, glucose 11g / L, sodium chloride ...
Embodiment 3
[0070] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:
[0071] 1) Preparation of primary strains:
[0072] a. Using an inoculation loop, pick a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae respectively and inoculate them into 250mL Erlenmeyer flask yeast culture medium filled with 100mL liquid yeast culture medium. Shake culture at 30°C for 36 hours until the concentration of each bacterial solution reaches 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;
[0073] in:
[0074] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 7.5.
[0075] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 9g / L, beef extract 4.5g / L, glucose 9g / L, sodium chloride ...
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