Preparation method of nontoxic cotton dreg animal feed

Abstract

The invention relates to a preparation method of a nontoxic cotton dreg animal feed, and aims to provide a preparation method of a nontoxic cotton dreg animal feed, which can be used for solving the problem of detoxication of cotton dregs and increasing nutritional substances in cotton dregs. According to technical key points, the method comprises the following steps of: (1) preparing a primary strain; (2) preparing a secondary strain; (3) preparing a fermentation substrate; and (4) inoculating the fermentation substrate, adjusting the water content to 45-50 percent, mixing uniformly, fermenting for 3-6 days, and drying. The nontoxic cotton dreg animal feed can be taken as a feed for livestock and aquatic livestock, and belongs to the technical field of preparation of feeds.

Description

technical field

[0001] The invention relates to a method for preparing animal feed, in particular to a method for preparing non-toxic cotton meal animal feed, which belongs to the technical field of feed. Background technique

[0002] my country is a large cotton-producing country, with an annual output of more than 6 million tons of cottonseed meal, ranking first in the world in total. Cottonseed meal, as a by-product of cottonseed pressing, is a cake obtained after cottonseed is pressed, and then most of the residual oil in it is separated through a leaching process to obtain a reddish or yellow granular product. . Cottonseed meal is a rich source of protein, containing 30% to 45% of crude protein, 11% to 15% of crude fiber, and more B vitamins, thiamine and organic phosphorus. At home and abroad, the use of cottonseed cakes to breed livestock, poultry and aquatic animals has achieved obvious economic benefits. However, because cottonseed meal contains gossypol, which h...

Examples

Embodiment 1

[0032] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:

[0033] 1) Preparation of primary strains:

[0034] a. Use an inoculation loop to inoculate a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae into a 250mL Erlenmeyer flask filled with 100mL liquid yeast culture medium, shake at 30°C Cultivate for 36 hours until the concentration of each bacterial solution is 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;

[0035] in:

[0036] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 7.0.

[0037] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 9g / L, beef extract 5.5g / L, glucose 11g / L, sodium chloride 4.5g / L, Ph 6.5.

[0038] The concentration of e...

Embodiment 2

[0051] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:

[0052] 1) Preparation of primary strains:

[0053] a. Using an inoculation loop, pick a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae respectively and inoculate them into 250mL Erlenmeyer flask yeast culture medium filled with 100mL liquid yeast culture medium. Shake culture at 30°C for 36 hours until the concentration of each bacterial solution reaches 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;

[0054] in:

[0055] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 6.5.

[0056] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 10g / L, beef extract 5g / L, glucose 11g / L, sodium chloride ...

Embodiment 3

[0070] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:

[0071] 1) Preparation of primary strains:

[0072] a. Using an inoculation loop, pick a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae respectively and inoculate them into 250mL Erlenmeyer flask yeast culture medium filled with 100mL liquid yeast culture medium. Shake culture at 30°C for 36 hours until the concentration of each bacterial solution reaches 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;

[0073] in:

[0074] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 7.5.

[0075] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 9g / L, beef extract 4.5g / L, glucose 9g / L, sodium chloride ...

technical field

[0001] The invention relates to a method for preparing animal feed, in particular to a method for preparing non-toxic cotton meal animal feed, which belongs to the technical field of feed. Background technique

[0002] my country is a large cotton-producing country, with an annual output of more than 6 million tons of cottonseed meal, ranking first in the world in total. Cottonseed meal, as a by-product of cottonseed pressing, is a cake obtained after cottonseed is pressed, and then most of the residual oil in it is separated through a leaching process to obtain a reddish or yellow granular product. . Cottonseed meal is a rich source of protein, containing 30% to 45% of crude protein, 11% to 15% of crude fiber, and more B vitamins, thiamine and organic phosphorus. At home and abroad, the use of cottonseed cakes to breed livestock, poultry and aquatic animals has achieved obvious economic benefits. However, because cottonseed meal contains gossypol, which h...

Examples

Embodiment 1

[0032] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:

[0033] 1) Preparation of primary strains:

[0034] a. Use an inoculation loop to inoculate a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae into a 250mL Erlenmeyer flask filled with 100mL liquid yeast culture medium, shake at 30°C Cultivate for 36 hours until the concentration of each bacterial solution is 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;

[0035] in:

[0036] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 7.0.

[0037] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 9g / L, beef extract 5.5g / L, glucose 11g / L, sodium chloride 4.5g / L, Ph 6.5.

[0038] The concentration of e...

Embodiment 2

[0051] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:

[0052] 1) Preparation of primary strains:

[0053] a. Using an inoculation loop, pick a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae respectively and inoculate them into 250mL Erlenmeyer flask yeast culture medium filled with 100mL liquid yeast culture medium. Shake culture at 30°C for 36 hours until the concentration of each bacterial solution reaches 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;

[0054] in:

[0055] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 6.5.

[0056] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 10g / L, beef extract 5g / L, glucose 11g / L, sodium chloride ...

Embodiment 3

[0070] A preparation method of non-toxic cotton meal animal feed, comprising the steps of:

[0071] 1) Preparation of primary strains:

[0072] a. Using an inoculation loop, pick a ring of Candida tropicalis, Candida utilis, and Saccharomyces cerevisiae respectively and inoculate them into 250mL Erlenmeyer flask yeast culture medium filled with 100mL liquid yeast culture medium. Shake culture at 30°C for 36 hours until the concentration of each bacterial solution reaches 10 8 ~10 9 cfu / mL, the prepared first-class strains of Candida, first-class strains of Candida utilis, and first-class strains of Saccharomyces cerevisiae;

[0073] in:

[0074] The concentration of each component in the yeast seed culture medium is: peptone 4.55g / L, yeast extract 14g / L, sodium chloride 3.5g / L, glucose 9g / L, and the pH value is 7.5.

[0075] The concentration of each component in the Bacillus subtilis seed culture medium is: peptone 9g / L, beef extract 4.5g / L, glucose 9g / L, sodium chloride ...