Method for extracting mulberroside A from white mulberry root-bark

Abstract

The invention belongs to the field of natural pharmaceutical chemistry and discloses a method for extracting mulberroside A from white mulberry root-bark. The method comprises the following steps of: 1) crushing the white mulberry root-bark, adding an ethanol solution at the concentration of between 30 and 90 percent in an amount which is 6 to 15 times that of the crushed mulberry root-bark, refluxing and extracting for 1 to 3 times, decompressing an extracting solution to recover ethanol, regulating the pH value to 4 to 5, standing and precipitating, and filtering to obtain a concentrated solution; 2) adding the concentrated solution into an ultrafiltration membrane and ultrafiltering, adding permeate into macroporous resin and absorbing, eluting by using an ethanol solution at the concentration of between 40 and 60 percent, collecting eluent, concentrating under reduced pressure, regulating the pH value of a concentrated solution to 4 to 5, filtering, and adding ethyl acetate and extracting to obtain extract liquor; and 3) recovering a reagent from the extract liquor, adding activated carbon and drying, loading into an activated carbon column, performing gradient elution by using ethanol solution, performing thin-layer detection, collecting target ingredients, concentrating, and drying at low temperature to obtain the mulberroside A. The process is easy to operate, is low in production cost and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a method for extracting mulberry side A from Cortex Mori. Background technique [0002] Morus alba is a plant of the family Moraceae Morus alba Dry root bark of L. Traditional medicine believes that Morus alba is sweet, cold, and returns to the lung meridian, and has the functions of purging the lungs and relieving asthma, promoting water and reducing swelling. It is often used to treat asthma and cough due to lung heat, edema, oliguria, and edema of the face and skin. Modern medical research shows that Morus alba contains coumarin, polyphenols, sugars, volatile oil and so on. Among them, mulberry glycoside A is the main component of relieving cough and relieving asthma in Morus alba. [0003] Mulberroside A (Mulberroside A ) is a polyhydroxystilbene compound with the molecular formula C 26 h 32 o 14 , with a molecular weight of 568, has the function...

AI Technical Summary

Problems solved by technology

These methods are cumbersome to operate, the process is complicated, the preparation amount is small, the preparation cycle is long, and it is difficult to realize industrialization