Method for extracting mulberroside A from white mulberry root-bark

A technology of Cortex Morus alba and Morus alba, applied in the field of natural medicinal chemistry, can solve the problems of small preparation amount, long preparation cycle, complex process, etc., and achieve the effect of reducing decomposition and facilitating subsequent purification

Inactive Publication Date: 2012-01-25
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are cumbersome to operate, the process is complicated, the preparation amount is small, the preparation cycle is long, and it is difficult to realize industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Morus alba cortex is crushed, 20kg and 6 times the amount of 30% ethanol solution are refluxed and extracted 3 times, each time for 1 hour, the combined extracts are decompressed to recover ethanol, the pH is adjusted to 5 with sodium dihydrogen phosphate, and the precipitate is placed, and the concentrated solution is filtered, and the intercepted solution is added. Ultrafiltration with hollow cellulose ultrafiltration membrane with a molecular weight of 2000, adding NKA-II macroporous resin to the permeated filtrate for adsorption, taking 6BV40% ethanol solution for elution, collecting the eluate and concentrating it under reduced pressure, and adjusting the pH of the concentrated solution to 4 with sodium dihydrogen phosphate, Filtrate and extract with ethyl acetate for 3 times to obtain the extract, recover the reagent from the extract, add 100g of granular activated carbon to dry, put it into an activated carbon column, elute impurities with 15BV50% ethanol, and then...

Embodiment 2

[0021] Grind Morus alba, take 20kg and add 15 times the amount of 90% ethanol solution to reflux and extract for 1 hour, extract the extract to recover ethanol under reduced pressure, adjust the pH to 4.5 with sodium dihydrogen phosphate and place the precipitate, filter to obtain the concentrated solution, add hollow Cellulose ultrafiltration membrane ultrafiltration, permeate the filtrate and add ADS-21 macroporous resin for adsorption, take 5BV60% ethanol solution for elution, collect the eluate and concentrate under reduced pressure, adjust the pH of the concentrated solution to 4 with sodium dihydrogen phosphate, filter and add acetic acid Ethyl ester was extracted 3 times to obtain the extract, the reagent was recovered from the extract, 200g of granular activated carbon was added to dry, and then loaded into an activated carbon column, 10BV70% ethanol was used to elute impurities, and then 95% ethanol was used to elute, TLC detection, and the target components were collec...

Embodiment 3

[0023] Grind Morus alba, take 20kg and add 8 times the amount of 60% ethanol solution to reflux for extraction for 1 hour, extract twice, extract the ethanol under reduced pressure, adjust the pH to 4 with sodium dihydrogen phosphate and place it for precipitation, filter to obtain the concentrated solution, add a molecular weight cut-off of 3000 Hollow cellulose ultrafiltration membrane ultrafiltration, permeate the filtrate and add LSA-7 macroporous resin for adsorption, take 5BV60% ethanol solution for elution, collect the eluate and concentrate under reduced pressure, the concentrated solution is adjusted to pH 4.5 with sodium dihydrogen phosphate, Filtrate and extract with ethyl acetate for 3 times to obtain the extract, recover the reagent from the extract, add 250g of granular activated carbon to dry, put it into an activated carbon column, elute the impurities with 10BV70% ethanol, then take 95% ethanol to elute, TLC detection, and collect The target components were con...

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PUM

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Abstract

The invention belongs to the field of natural pharmaceutical chemistry and discloses a method for extracting mulberroside A from white mulberry root-bark. The method comprises the following steps of: 1) crushing the white mulberry root-bark, adding an ethanol solution at the concentration of between 30 and 90 percent in an amount which is 6 to 15 times that of the crushed mulberry root-bark, refluxing and extracting for 1 to 3 times, decompressing an extracting solution to recover ethanol, regulating the pH value to 4 to 5, standing and precipitating, and filtering to obtain a concentrated solution; 2) adding the concentrated solution into an ultrafiltration membrane and ultrafiltering, adding permeate into macroporous resin and absorbing, eluting by using an ethanol solution at the concentration of between 40 and 60 percent, collecting eluent, concentrating under reduced pressure, regulating the pH value of a concentrated solution to 4 to 5, filtering, and adding ethyl acetate and extracting to obtain extract liquor; and 3) recovering a reagent from the extract liquor, adding activated carbon and drying, loading into an activated carbon column, performing gradient elution by using ethanol solution, performing thin-layer detection, collecting target ingredients, concentrating, and drying at low temperature to obtain the mulberroside A. The process is easy to operate, is low in production cost and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a method for extracting mulberry side A from Cortex Mori. Background technique [0002] Morus alba is a plant of the family Moraceae Morus alba Dry root bark of L. Traditional medicine believes that Morus alba is sweet, cold, and returns to the lung meridian, and has the functions of purging the lungs and relieving asthma, promoting water and reducing swelling. It is often used to treat asthma and cough due to lung heat, edema, oliguria, and edema of the face and skin. Modern medical research shows that Morus alba contains coumarin, polyphenols, sugars, volatile oil and so on. Among them, mulberry glycoside A is the main component of relieving cough and relieving asthma in Morus alba. [0003] Mulberroside A (Mulberroside A ) is a polyhydroxystilbene compound with the molecular formula C 26 h 32 o 14 , with a molecular weight of 568, has the function...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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