A medical composition for treating breast cancer

A composition and medical technology, applied in drug combinations, anti-tumor drugs, antibodies, etc., can solve the problem of low 5-year survival rate of patients with advanced breast cancer

Active Publication Date: 2012-02-08
LBL BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early-stage breast cancer responds well, but 5-year survival

Method used

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  • A medical composition for treating breast cancer
  • A medical composition for treating breast cancer
  • A medical composition for treating breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0169] Example 1: Anti-interleukin-19 antibody 1BB1 inhibits proliferation of breast cancer cells

[0170] Human MCF-7 and mouse 4T1 breast cancer cell lines were cultured for 16 hours in medium containing fetal bovine serum. Next, the 2×10 5 MCF-7 or 4T1 cells were planted and cultured in a medium containing 2% FBS for 24 hours, wherein this medium contained PBS, human interleukin-19 (for MCF-7 cells, the content was 200 or 400 μg / mL), mouse interleukin-19 (200 or 400 μg / mL for 4T1 cells), antibody 1BB1 (8 μg / mL), or a mixture of interleukin-19 and antibody 1BB1 (1:10) . Then, the cells were placed in a solution containing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (3-(4,5-Dimethylthiazol-2-yl )-2,5-diphenyltetrazolium bromide, MTT) (1 mg / ml; Sigma-Aldrich) for 3 hours. Dimethyl sulfoxide (DMSO) was added to the incubator, and the absorbance was measured at 500 nm. Here, FBS (10%) (Sigma-Aldrich) was used as a positive control group.

[0171] As shown...

Embodiment 2

[0172] Example 2: Treatment of breast cancer with antibody 1BB1

[0173] The effect of anti-interleukin-19 antibody 1BB1 on the survival of breast cancer-bearing mice was studied as follows. Mouse breast cancer cell line 4T1 was cultured under appropriate conditions, collected, and resuspended in PBS to obtain a final concentration of 2×10 5 / 100 μL. First, 6-week-old female BALB / c mice were given pentobarbital (pentobarbital) for general anesthesia (given at a rate of 50 mg / kg of mouse body weight), and then 4T1 cells (2×10 5 / 100μL) with a syringe (29gauge, BD Ultra-Fine; Becton Dickinson), and directly injected into the left ventricle (left ventricle) of 6-week-old female BALB / c mice. Each mouse was randomly divided into three groups (n=6 / group), and each group was injected with vehicle (PBS), control mouse antibody (mIgG, 10 mg / kg), or antibody 1BB1 (10 mg / kg) three times a week. kg). Mice not injected with 4T1 cells served as healthy controls. The survival rates of t...

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PUM

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Abstract

The invention relates a medical composition using an anti-IL-19 antibody for treating breast cancer, either alone or in combination with an anti-IL-20 and/or anti-IL-20R1 antibody.

Description

technical field [0001] The invention relates to the application of an anti-interleukin-19 antibody in treating breast cancer. Background technique [0002] Breast cancer, such as ductal carcinoma and lobular carcinoma, is one of the most common cancers in women. Among them, one in eight women will develop breast cancer. [0003] Treatments for breast cancer include chemotherapy, radiation therapy, and surgery. The treatment effect of early breast cancer is good, but the 5-year survival rate of patients with advanced breast cancer is less than 50%. [0004] Therefore, there is still a need to develop a novel treatment method for breast cancer, in order to show a very high therapeutic effect in various stages of cancer. Contents of the invention [0005] The present invention finds that the monoclonal antibody 1BB1 specific to human interleukin-19 (IL-19) can effectively inhibit the proliferation of breast cancer cells and the growth of breast cancer, and prolong the life...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61P35/00A61P35/04
CPCA61K2039/505C07K16/2803A61P35/00A61P35/04
Inventor 张明熙
Owner LBL BIOTECH INC
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