Reagent kit for identifying xanthomonas campestris pv. campestris No.4 physiological race
A technology of Xanthomonas rapeseed and physiological races, which is applied in the directions of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve problems such as reports on physiological races of unseen bacteria, shorten identification time, overcome identification inefficient effect
Inactive Publication Date: 2012-11-21
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology
Studies on the pathogenicity differentiation of cruciferous vegetable black rot pathogens in my country are relatively common, but there are no relevant reports on the physiological races of this fungus
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Embodiment 1
[0015] Example 1. Primers for identification of Xanthomonas campestris pv. campestris No. 4 physiological race and its application
[0016] 1. Acquisition of primers for identification of race 4 of Xanthomonas campestris pv. campestris
[0017] The AFLP marker (amplified fragment length polymorphism technique) method was used to analyze the six physiological race standard strains, and the AFLP fingerprints of the six physiological race standard strains were obtained, and the specific stripes of the fourth physiological race standard strain were analyzed. The band was recovered to obtain a unique DNA fragment of the No. 4 physiological race. The DNA fragment was sequenced, and primers were designed according to the obtained sequence, and the obtained primer was the primer Pxc4 (abbreviation of Primer Xcc4) of the present invention. The primer sequences are: the upstream forward primer sequence is: 5'-GAAGCTCCTCGCGGAAATG-3 (sequence 1 in the sequence listing), the downstream rev...
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The invention discloses a pair of primers for the auxiliary identification of xanthomonas campestris pv. campestris No.4 physiological race and an application thereof. The nucleotide sequences of the pair of primers are respectively sequences 1 in a sequence table and sequences 2 in the sequence table. The pair of primers can be used for carrying out the physiological race identification on xanthomonas campestris pv. campestris bacterial strains, and the identification time is shortened, so the xanthomonas campestris pv. campestris No.4 physiological race can be fast identified. The method for identifying the physiological race by the primers solves the problems that a host identification method of the traditional physiological race identification method is easily interfered by the environment factor and the long growing period of identification hosts, so the identification efficiency is low, and the like.
Description
technical field [0001] The invention relates to a pair of primers for auxiliary identification of No. 4 physiological race of Xanthomonas campestris pv. campestris and application thereof. Background technique [0002] Xanthomonas campestris pv. campestris has a phenomenon of physiological race differentiation, and it is commonly reported in the world that it is divided into six races. Currently, Xanthomonas campestris pv. Most of the physiological race identification of Xanthomonas campestris pv. campestris races and related pathovars. Phytopathology 91: 492-499. Yan Hong, Qiu Jiyan, Zhang Taotao, Tian Zhaofeng. 2001. Research on multi-resistance identification method for co-inoculation of three pathogens in Chinese cabbage seedling stage. Acta Phytopathology, 31(3): 109-113.). Conventional host identification methods based on physiological race identification have disadvantages such as long identification time and great influence by environmental factors, so it is urgent ...
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IPC IPC(8): C12N15/11C12Q1/68C12Q1/04C12R1/64
Inventor 严红燕继晔翟文慧刘凡林秀敏
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES